Tumor Necrosis Factor Alpha （TNFa） Protein-肿瘤坏死因子a （TNFa）蛋白操作规程
P90133Hu01
Tumor Necrosis Factor Alpha （TNFa）
Organism: Homo sapiens （Human）
 

[ DESCRIPTION ]
Protein Names: Tumor Necrosis Factor Alpha
Synonyms: TNFa, TNFSF2
Species: Human
Size: 100μg
Source: Escherichia coli-derived
Subcellular Location: Cell membrane; Single-pass type II membrane protein,
Secreted.
[ PROPERTIES ]
Residues: Val77~Leu233 （Accession # P01375）, with two N-terminal Tags,His-tag and
T7-tag.
Grade & Purity: >95%, 21 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Supplied as liquid form in Phosphate buffered saline（PBS）,pH 7.4.
Endotoxin Level: <1.0 EU per 1μg （determined by the LAL method）.
Applications: SDS-PAGE; WB; ELISA; IP.
（May be suitable for use in other assays to be determined by the end user.）
Predicted Molecular Mass: 21.2 kDa
Predicted isoelectric point: 7.9
[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate ofthe target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubatethe protein at 37oC for 48h, and no obvious
degradation and precipitation were observed. （Referring from ChinaBiological Products Standard, which was
calculated by the Arrhenius equation.） The loss of this protein is lessthan 5% within the expiration date under
appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag andT7-tag, its sequence is listed below.
MGSSHHHHHHSSGLVPRGSHMASMTGGMGRGSEF-VRSS SRTPSDKPVA HVVANPQAEGQLQWLNRRAN ALLANGVELR
DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRETPEGAEAKPW YEPIYLGGVF
QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL