一、人β干扰素 ELISA试剂盒的产品简述：

       ELISA 品  牌： USCN

       ELISA 货  号：070222h

       ELISA英文名：human IFNb ELISAKit

       ELISA中文名：人β干扰素,IFNb ELISA 试剂盒

二、人IFNb ELISAKIT的技术参数：
      1. 检测范围：

      2. 灵  敏  度：

      3. 适用标本：

      4.保存条件：2-8℃

       5. 有 效  期： 6个月

三、human IFNb ELISA试剂盒的产品用途：
      产品用途：酶联免疫实验（ELISA实验）

      备     注：仅供参考，请以随货纸质说明书为准，更多产品如抗体（antibody）；蛋白（protein）；鲎试剂；内毒素工作标准品；内毒素指示剂；无热原检查用水；无热原吸咀；紫外灯；色谱仪；色谱柱等更详细信息请登录上海武昊公司www.wuhaosh.com 或致电咨询

    
 ELISA实验前，血清、血浆、组织、细胞培养上清等样品的采集及处理

 Serum - Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximay 1000×g. Assay freshly prepared serum immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at  000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Tissue homogenates  - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS（0.02mol/L,pH 7.0-7.2） to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in 5-10 mL of PBS with a glass homogenizer on ice（Micro Tissue Grinders woks, too）. The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 5 minutes at 5000×g. Remove the supernate and assay immediay or aliquot and store at ≤-20oC.

Cell culture supernates    - Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.