To determine effectors of cell signaling pathways in the context of intact functioning cells, cell-based reporter assays are an ideal option. Using luciferase reporters downstream of a pathway-specific promoter response element, signaling activity can be conveniently measured by the output of luciferase activity. The system can be applied across a wide variety of cell types and signaling pathways and allows for high throughput screening of large libraries of compounds for inhibitory or activating function. Alternatively, fluorescent proteins such as eGFP and RFP can be used to visualize live cellular readouts.

We offer rapid and dependable screening and profiling services for cell signaling pathway effectors. Our team of experts, using our broad portfolio of in-house developed cell-based assay kits, can provide:

• Screening for active compounds (small molecules, peptides, or antibodies)

• Determination of IC₅₀ or EC₅₀

• Project guidance and questions answered in a timely manner

• Delivery of detailed results promptly and on time

If your desired cell-based assay is not available, we may be able to develop a custom product for you.

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Available Service Assays

AP1 Reporter (JNK Pathway)	NFAT Reporter (Jurkat)
ARE Reporter (Nrf2 Antioxidant Pathway)	Notch1/CSL Reporter (Notch Signaling Pathway)
CREB/CRE Reporter (cAMP/PKA Pathway)	Myc Reporter (Myc Signaling Pathway)
FOXO Reporter (PI3K/AKT Pathway)	RARα Reporter
Gli Reporter (Hedgehog Pathway)	RARβ Reporter
IRF Reporter (cGAS-Sting Signaling Pathway)	RARγ Reporter
ISRE Reporter (JAK Pathway)	SBE Reporter (TGF/SMAD Signaling Pathway)
NF-κB Reporter (A549)	SRE Reporter (ERK Pathway)
NF-κB Reporter (HCT-116)	STAT3 Reporter
NF-κB Reporter (HEK293)	TEAD Reporter (Hippo Pathway)

NF-κB Reporter (Jurkat)	TCF/LEF Reporter (Wnt Signaling Pathway)
NF-κB Reporter (NIH-3T3)	NF-κB Reporter (THP-1)

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