15
CRL-2549 Panc 03.27 人胰腺癌细胞
ATCC® Number: CRL-2549™ Price: $417.00
Designations: Panc 03.27
Depositors: EM Jaffee
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: epithelial
Source: Organ: pancreas
Disease: adenocarcinoma
Cellular Products: cytokeratins 7 and 18
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2549 Panc 03.27 人胰腺癌细胞
Tumorigenic: Yes
Oncogene: K-ras + （wild-type）
Antigen Expression: MHC class I +; MHC class II - [50655]
Blood type A; Rh+
DNA Profile （STR）: Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12
D5S818: 12,13
D7S820: 8,13
THO1: 6
TPOX: 8,9
vWA: 16,17
Age: 65 years
Gender: female
Ethnicity: White
Comments: Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37C.
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 37.5 hrs
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001
recommended serum:ATCC 30-2020
0.25% （w/v） Trypsin - 0.53 mM EDTA in Hank' BSS （w/o Ca++, Mg++）:ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602
CRL-2549
