CRL-2119 HPAC 人胰腺癌细胞

ATCC® Number: CRL-2119™ Price: $323.00

Designations: HPAC

Depositors: WR Gower

Biosaum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

Source: Organ: pancreasfety Level: 1

Shipped: frozen

Medium & Ser

Disease: adenocarcinoma

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CRL-2119 HPAC 人胰腺癌细胞

Isolation: Isolation date: 1985

Receptors: epidermal growth factor （EGF）, expressed

glucocorticoid, expressed

epidermal growth factor （EGF）; glucocorticoid

Tumorigenic: Yes

DNA Profile （STR）: Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 9,10

D5S818: 12

D7S820: 10,12

THO1: 9.3

TPOX: 10,11

vWA: 15,17

Cytogenetic Analysis: modal number = 61

Age: 64 years

Gender: female

Ethnicity: Caucasian

Comments: HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.

HPAC proliferation is stimulated by insulin, insulin-like growth factor I （IGF-I）, epidermal growth factor （EGF）, and transforming growth factor alpha （TGF alpha）.

Cell growth is suppressed by dexamethasone and other glucocorticoids.

HPAC cells are positive for keratin and negative for vimentin and chromogranin A.

Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1.

Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.

In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.

HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 41 hrs

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2006

recommended serum:ATCC 30-2020

References: 22987: Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.

CRL-2119