MSU誘導(dǎo)的腹膜炎模型
- 公司名稱 上海宇玫博生物科技有限公司
- 品牌
- 型號
- 產(chǎn)地 上海
- 廠商性質(zhì) 生產(chǎn)廠家
- 更新時間 2017/8/7 10:27:01
- 訪問次數(shù) 683
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實驗方案:
1. MSU用PBS重懸, 每只實驗鼠腹腔注射1mgMSU;
2. 6小時后取腹腔灌洗液, 用10mL PBS沖洗;
3. 分析灌洗液中中性粒細(xì)胞數(shù)量,流式細(xì)胞檢測,細(xì)胞表面分子: Ly-6G和CD11b
Brief protocol of MSU induced mouse peritonitis model
1. Mice were i.p. injected of 1mg MSU crystals in 0.5 ml PBS;
2. 6h later, animals were killed by CO2 exposure;
3. Peritoneal cavities were washed with 10 ml of PBS;
4. The lavage fluids were analyzed for neutrophil recruitment by fluorescence-activated cell sorting using the neutrophil markers Ly-6G and CD11b