Mouse IL-1 beta ELISA Kit INTRODUCTION
Monocytes are the main source of secreted IL-1. They express predominantly IL-1 beta while human keratinocytes express large amounts of IL-1 alpha. IL-1 is produced also by activated macrophages from different sources （alveolar macrophages, Kupffer cells, adherent spleen and peritoneal macrophages） and also by peripheral neutrophil granulocytes. IL-1apha and IL-1 beta are biologically more or less equivalent pleiotropic factors that act locally and also systemically. IL-1 beta is a potent immunomodulator, which mediates a wide range of immune and inflammatory responses including the activation of B and T cells.

Mouse IL-1 beta ELISA （Enzyme-Linked Immunosorbent Assay） kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 beta cell lysate and tissue lysate. This assay employs an antibody specific for mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed,a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, Mouse IL-1 beta ELISA Kit and the intensity of the color is measured at 450nm。

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