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The Optosplit III has been designed as a convenient, inexpensive solution for simultaneous imaging. Splitting is usually performed on the basis of wavelength or polarization, allowing applications where there is a requirement for simultaneous or high speed acquisition of multiple emission bands or polarization states. The simultaneous acquisition of up to three images offers a major benefit over manual or electronic filter changers, as there is no longer a need to pause acquisition while the filter position is changed. This allows your camera to be operated at the very fastest capture rates it is capable of achieving.

The Optosplit III is usually supplied with unity magnification and fitted with a rectangular aperture to define the ROI. It includes controls to allow up to three images to be positioned accurately and conveniently within the camera frame. Device drivers are included in several commercial imaging packages to assist registration and to allow real-time and off-line ratioing or image overlays. Whilst optimized for coupling to a scientific microscope, the Optosplit III can also be used with camera lenses or any other system of lenses that produce an image plane of suitable size.