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Capture and separation of l-histidine through optimized zinc-decorated magnetic silica spheres
閱讀：280 發(fā)布時(shí)間：2018-4-4a
Centro de Física, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
b
CMEMS-UMinho, Universidade do Minho, DEI, Campus de Azurém, Guimarães 4800-058, Portugal
c
Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I + D, C/Poeta Mariano Esquillor S/N, 50018 Zaragoza, Spain
d
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine CIBER-BBN, Centro de Investigación Biomédica en Red, C/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain
e
Centro/Departamento de Química, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
f
BCMaterials, Parque Científico y Tecnológico de Bizkaia, 48160 Derio, Spain
g
IKERBASQUE, Basque Foundation for Science, 48013, Bilbao, Spain
摘要：Zinc-decorated magnetic silica spheres were developed, optimized and tested for the capture and separation of l-histidine. The magnetic silica spheres were prepared using a simple sol-gel method and show excellent magnetic characteristics, adsorption capacity toward metal ions, and stability in aqueous solution in a wide pH range. The binding capacity of zinc-decorated magnetic silica spheres to histidine proved to be strongly influenced by the morphology, composition and concentration of metal at the surface of the magnetic silica spheres and therefore these parameters should be carefully controlled in order to maximize the performance for protein purification purposes. Optimized zinc-decorated magnetic silica spheres demonstrate a binding capacity to l-histidine of approximay 44 mg g−1 at the optimum binding pH buffer.