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Rapid analysis of malachite green and leucomalachite green in fish muscles with surface-enhanced res

閱讀：314 發(fā)布時間：2018-01-16

Surface-enhanced resonance Raman scattering (SERRS) coupled with gold nanospheres was applied for rapid analysis of the hazardous substances malachite green (MG) and leucomalachite green (LMG) in fish muscle tissues. The lowest concentration of MG that could be detected was 0.5 ng mL-1 with high linear correlation (R2= 0.970–0.998) between MG concentration and intensities of characteristic Raman peaks. A simplified sample preparation method taking less than 1 h for recovering MG and LMG in fish fillets was developed for SERRS analysis, and 4–8 samples could be handled in parallel. MG and LMG could be detected in extracts of tilapia fish fillets at as low as 2 ng g-1 with SERRS and a simple principle component analysis method. For six other fish species, the lowest detectable concentration of MG ranged from 1 ng g-1 to 10 ng g-1. This study provides a new sensitive approach for the detection of trace amounts of the prohibited drugs MG and LMG in muscle food, which has the potential for rapidly screening a large number of samples.（表面增強(qiáng)的共振拉曼散射(SERRS)和金納米球被用于快速分析魚類肌肉組織中有害物質(zhì)的有害物質(zhì)(MG), (LMG),(LMG)。一種簡單的樣品制備方法被開發(fā),利用SERRS技術(shù)不到1小時時間從魚塊中回收MG and LMG,4-8個樣品可以同時平行試驗(yàn)。用SERRS在羅非魚魚塊提取物中檢測到MG and LMG濃度2 ng g-1,其他六種魚塊MG的zui低檢測濃度從1 ng g-1到10 ng g-1。這項(xiàng)研究提供了一種新的靈敏方法,用于檢測肌肉食品中違禁藥物MG和LMG的微量含量,這種方法可以迅速篩選大量的樣本。）

提供商	上海安譜實(shí)驗(yàn)科技股份有限公司	下載次數(shù)	75次
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Rapid analysis of malachite green and leucomalachite green in fish muscles with surface-enhanced res

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