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Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the
5′ → 3′ polymerase activity, but lacks 5′ →3′ exonuclease activity. It can be use to isothermal amplification （LAMP）, DNA sequencing through high GC regions and Rapid Sequencing from nanogram amounts of DNA template.