别称

SNU387; NCI-SNU-387

种属

人类

年龄（性别）

女性，41岁

组织来源

肝癌

生长特性

贴壁细胞

细胞形态

上皮细胞样

背景描述

SNU-387 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.

生物安全等级

2

生长培养基

RPMI-1640＋10% FBS＋1% P/S

推荐传代比例

1:3-1:6

推荐换液频率

2~3次/周

倍增时间

~61小时 (PubMed=7543080)

冻存条件

冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮

培养条件

气相：空气，95%；CO2，5%温度：37℃

保藏机构

ATCC; CRL-2237