Thermo Scientific PageRuler Plus 预染蛋白分子量标准是九种蓝色、橙色和绿色染色蛋白(10 至 250 kDa)的混合物,可用作蛋白电泳 (SDS-PAGE) 和 Western 印迹中的分子量标准品。蛋白分子量标准品以即用型规格提供,用于直接上样至凝胶;使用前无需加热、还原或添加上样缓冲液。
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应用 ? 在 SDS-聚丙烯.酰胺凝胶电泳期间监测蛋白迁移 ? Western 印迹后监测蛋白转印到膜上 ? SDS-聚丙烯.酰胺凝胶和 Western 印迹上的蛋白大小分析
PageRuler™ Plus 预染蛋白分子量标准内容与储存 内容物:两小瓶,250 µL/瓶 储存缓冲液:62.5 mM Tris-H3PO4(25°C 下 pH 值 7.5)、1 mM EDTA、2% SDS、10 mM DTT、1 mM NaN3和 33% 甘油 储存:接收后,在 -20°C 下储存
Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
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Applications ? Monitoring protein migration during SDS-polyacrylamide gel electrophoresis ? Monitoring protein transfer onto membranes after western blotting ? Sizing of proteins on SDS-polyacrylamide gels and western blots