Ca761 小鼠乳腺癌細(xì)胞
Catalogue No.: C1429
Product Format: a T25 flask
Culture Properties：貼壁
Complete Growth Medium: 89%1640+10%FBS+1%雙抗
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Application: Cells and cancer research
NOTE: FOR RESEARCH USE ONLY.

Components

Operation steps for cell culture

1. 吸走用于培養(yǎng)基，加入3-5mL PBS后輕輕晃動(dòng)培養(yǎng)瓶潤(rùn)洗細(xì)胞；
2. 吸干凈PBS后加入1mL 0.25%-0.53mM EDTA，輕輕搖晃培養(yǎng)皿使浸沒細(xì)胞表面，培養(yǎng)瓶放37度培養(yǎng)箱消化；
   （細(xì)胞對(duì)于消化比較敏感，消化過度會(huì)嚴(yán)重影響細(xì)胞狀態(tài)，導(dǎo)致細(xì)胞傳代死亡漂浮或者傳代后不長(zhǎng)。細(xì)胞消化到細(xì)胞間隙變大但未脫落，可以輕輕吹下時(shí)即可終止，禁止消化到細(xì)胞漂浮?；靹蚣?xì)胞時(shí)盡量輕柔，不要吹出大量氣泡。）
3. 加入6-8ml培養(yǎng)基終止消化，輕輕吹下細(xì)胞混勻；
4. 將混勻的細(xì)胞1000rpm（約150g）離心3min，棄上清，再用新鮮培養(yǎng)基重懸37度培養(yǎng)箱繼續(xù)培養(yǎng)；

傳代比例: 不同細(xì)胞生長(zhǎng)速度不一，具體傳代比例視細(xì)胞生長(zhǎng)速度而定，大部分細(xì)胞適用1:3-1:4傳代，生長(zhǎng)較慢的細(xì)胞可以1:2傳代。

Methods A total of 200 schizophrenic patients admitted to the hospital from July 2019 to June 2022 and 200 normal people who underwent physical examination were selected as the odkervation group and control group ，