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Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.

產(chǎn)品簡(jiǎn)介

Species ：Human

Protein Construction ：Expressed with an N-terminal Met.PDGF-BB (Ser82-Thr190)

Accession # P01127

Purity： > 97% as analyzed by SDS-PAGE

> 97% as analyzed by HPLC

Endotoxin Level： < 1 EU/μg of protein by LAL method

Biological Activity ：Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3.0 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.

Expression System： E. coli

Theoretical Molecular Weight ：24.8 kDa

Formulation： Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Reconstitution ：It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.

Storage & Stability： Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.

技術(shù)背景

Target Background ：Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.

Synonyms ：PDGFBB; IBGC5; PDGF-2; PDGF2; SIS; SSV; c-sis; platelet derived growth factor subunit B

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.