登革热NS1抗原检测试纸

 产品规格：25T/盒； 

 保存条件：避光 4-30度 保存。

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登革热NS1抗原检测试纸

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登革热NS1抗原检测试纸
 

9.      Pipette 100 µl streptavidin-HRP (ELISA kits) or anti-rabbit IgG-HRP (phosphoELISA™ kits) solution to each well except the chromogen blank(s).
10.  Cover wells with the plate cover and incubate for 30 minutes at room temperature.
11.  Thoroughly aspirate or decant solution from wells and discard the liquid. Wash wells 4 times.
12.  Pipette 100 µl of Stabilized Chromogen to each well. The liquid in the wells will begin to turn blue.
13.  Incubate for 30 minutes at room temperature and in the dark. Note: Do not cover the plate with aluminum foil or metalized mylar. The incubation time for chromogen substrate is often determined by the microtiter plate reader used. Many plate readers have the capacity to record a maximum optical density (OD) of 3.0. The OD values should be monitored and the substrate reaction stopped before the OD of the positive wells exceed the limits of the instrument. The OD values at 450 nm can only be read after the Stop Solution has been added to each well. If using a reader that records only to 3.0 OD, stopping the assay after 20 to 25 minutes is suggested.
14.  Pipette 100 µl of Stop Solution to each well. Tap gently on the side of the plate to mix. The solution in the wells should change from blue to yellow.

9。管啊100µL亲和斋（ELISA试剂盒）或者抗兔IgG抗体（phosphoelisa™套件）解决方案呀个个除咗咸湿空白（S）。
10。起与起威尔斯，哺育半个钟喺室温。
11。*吸出或者倒泻溶液由威尔斯同废液。洗威尔斯4次。
12。管啊100µL稳定发色团喺每。威尔斯体内嘅液体开始变蓝。
13。哺育半个钟，喺室温下，喺鼆中。注：唔包板用铝箔或者金属化聚酯薄膜。对咸湿底物哺育时间往往系由读者用微孔板。好多平板阅读器能够纪录3嘅zui大光密度（OD）。喺正向威尔斯嘅OD值超过仪器嘅极限前，应监测OD值同底物反应闸住。只有喺每井加闸住液后先可以读取450 nm处嘅OD值。如果使用只纪录到3 OD嘅读取器，建议喺二十到25分钟后闸住检测。
14。管啊100µl闸住解决每。细仔噉喺碟嘅一路细仔噉搅拌。威尔斯中嘅溶液应该由蓝变为黄色。