IgA(免疫球蛋白A)

广州健仑生物科技有限公司

IgA可作为免疫球蛋白IgA的α 链参考依据，可用于淋巴瘤、浆细胞瘤和B细胞来源的何杰金氏淋巴瘤的研究。也可用于肾小球肾炎的功能性分类和一些免疫性疾病的研究。

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IgA(免疫球蛋白A)

【产品介绍】

细胞定位：细胞浆

适用组织：石蜡/冰冻

阳性对照：扁桃体

抗原修复：热修复（柠檬酸）

抗体孵育时间：30-60min

IgA(免疫球蛋白A)

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【公司名称】 广州健仑生物科技有限公司
【市场部】     欧

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【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

首先作者以普通大鼠肾脏细胞为研究对象，发现这些细胞在迁移的过程中会不断地向胞外释放内容物，这种新发现的细胞事件被称为迁移胞吐作用(migracytosis)。细胞在迁移的过程中会将其收缩纤维留在胞体后侧。在收缩纤维的横截面处会有很多直径约为3um的囊泡，即为新发现的"迁移小体"。这些迁移小体中还包括有很多直径约50~100nm的小泡。zui终收缩纤维会发生断裂，从而这些迁移小体会释放到胞外并被周围的细胞吞噬。
由于之前没有任何相关的报道，为了进一步研究，需要进行纯化。作者通过密度梯度离心的方法得到了细胞裂解物的不同组分，比较发现migrasome富集在某一特定的组分中。之后，作者通过质谱的手段筛选了一系列migrasome特异性的蛋白，在大量膜蛋白与细胞骨架相关蛋白的候选物中，作者发现Tetraspanin-4(TSPAN4)是其中migrasome定位zui准确的蛋白质，因此作者人工构建了(TSPAN4)-荧光报告基因供后续研究。进一步研究发现：当细胞密度很大时，TSPAN4在细胞内的分布十分弥散，只有在细胞膜上有少量富集;但是当细胞密度较低时，随着细胞收缩纤维的伸长，很多TSPAN4富集于收缩纤维区域，特别是其表面的环状结构。之后，作者通过比较共聚焦显微镜成像结果与电镜投射结果，证实了这部分"环状"的结构即之前发现的migrasome。

First of all, the author used ordinary rat kidney cells as the research object and found that these cells continuously release the contents to the outside during the process of migration. The newly discovered cell event is called migracytosis. Cells migrate their contractile fibers to the back of the cell body during migration. There are many vesicles about 3um in diameter at the cross-section of the deflated fibers, the newly discovered "migrating bodies." These migration bodies also include many small bubbles about 50 ~ 100nm in diameter. The final defibrillated fibers break and the migrating bodies release to the extracellular and are swallowed by the surrounding cells.
Since there was no previous report, for further study, purification was required. The authors obtained different components of cell lysates by density gradient centrifugation, and found that migrasome is enriched in a particular component. After that, the authors screened a series of migrasome-specific proteins by mass spectrometry. In a large number of candidates for membrane proteins and cytoskeleton-associated proteins, the authors found that Tetraspanin-4 (TSPAN4) is the most accurate protein in which migrasome is located, The (TSPAN4) -fluorescent reporter gene was constructed manually for later study. Further studies showed that when the cell density is very high, the distribution of TSPAN4 in the cell is very diffuse, and only a small amount of enrichment is found in the cell membrane. However, when the cell density is low, many TSPAN4 are enriched with the cell shrinkage fiber elongation In the area of ??constricted fibers, especially the surface of the ring structure. Afterwards, the authors confirmed the "ring-like" structure of migrasome, which was previously found by comparing confocal microscopy imaging with electron microscopic projection results.