CD30(Ki-1抗原)鼠单克隆抗体

广州健仑生物科技有限公司

CD30 是一种分子量为120KDa的跨膜单链糖蛋白，是细胞因子配体CD30L的受体，在淋巴细胞活化中起重要作用。CD30表达于活化的 T/B 细胞、R-S细胞、部分淋巴滤泡周围的转化细胞、大多数间变性大细胞淋巴瘤细胞，在胚胎性癌中也发现CD30表达。此抗体主要用于研究R-S细胞和大多数间变性大细胞淋巴瘤。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【产品介绍】

细胞定位：细胞膜

克隆号：Ber-H2

同型：IgG1/K

适用组织：石蜡/冰冻

阳性对照：霍奇金氏淋巴瘤

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

CD30(Ki-1抗原)鼠单克隆抗体

（一）实验原理
双缩脲法（Biuret法）和Folin―酚试剂法（Lowry法）的明显缺点和许多限制，促使科学家们去寻找更好的蛋白质溶液测定的方法。
1976年由Bradford建立的考马斯亮兰法（Bradford法），是根据蛋白质与染料相结合的原理设计的。这种蛋白质测定法具有超过其他几种方法的突出优点，因而正在得到广泛的应用。这一方法是目前灵敏度zui高的蛋白质测定法。
考马斯亮兰G-250染料，在酸性溶液中与蛋白质结合，使染料的zui大吸收峰的位置（lmax），由465nm变为595nm，溶液的颜色也由棕黑色变为兰色。经研究认为，染料主要是与蛋白质中的碱性氨基酸（特别是精氨酸）和芳香族氨基酸残基相结合。
在595nm下测定的吸光度值A595，与蛋白质浓度成正比。
Bradford法的突出优点是：
（1）灵敏度高，据估计比Lowry法约高四倍，其zui低蛋白质检测量可达1mg。这是因为蛋白质与染料结合后产生的颜色变化很大，蛋白质－染料复合物有更高的消光系数，因而光吸收值随蛋白质浓度的变化比Lowry法要大的多。
（2）测定快速、简便，只需加一种试剂。完成一个样品的测定，只需要5分钟左右。由于染料与蛋白质结合的过程，大约只要2分钟即可完成，其颜色可以在1小时内保持稳定，且在5分钟至20分钟之间，颜色的稳定性。因而*不用像Lowry法那样费时和严格地控制时间。
（3）干扰物质少。如干扰Lowry法的K+、Na+、Mg2+离子、Tris缓冲液、糖和蔗糖、甘油、巯基乙醇、EDTA等均不干扰此测定法。

CD30(Ki-1抗原)鼠单克隆抗体

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

(A) experimental principle
The obvious shortcomings and many limitations of the Biuret and Folin-Phenol methods (Lowry's) have prompted scientists to find ways to better determine protein solutions.
The Bradford method, established by Bradford in 1976, is based on the combination of protein and dye. This protein assay has many outstanding advantages over several other methods and is therefore being widely used. This method is currently the most sensitive protein assay.
Coomassie brilliant blue G-250 dye, combined with the protein in acidic solution, so that the dye maximum absorption peak position (lmax) from 465nm to 595nm, the solution color from brown to blue. The study suggests that the dye is mainly combined with the basic amino acids in the protein (especially arginine) and aromatic amino acid residues.
The absorbance value A595 measured at 595 nm is proportional to the protein concentration.
The outstanding advantages of the Bradford method are:
(1) High sensitivity, which is estimated to be four times higher than the Lowry method, with a minimum protein detection of 1 mg. This is because the color produced by the binding of the protein to the dye changes greatly, and the protein-dye complex has a higher extinction coefficient, so that the light absorption value changes more strongly with the protein concentration than the Lowry method.
(2) Determination of fast, simple, just add a reagent. To complete a sample determination, only about 5 minutes. Due to the dye-protein binding process, it takes only about 2 minutes to complete and its color can be stable in less than 1 hour with the best color stability between 5 minutes and 20 minutes. Thus there is absoluy no time and strict time control required by the Lowry method.
(3) less interfering substances. Such interference with the Lowry method K +, Na +, Mg2 + ions, Tris buffer, sugar and sucrose, glycerol, mercaptoethanol, EDTA, etc. do not interfere with this assay.