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BRD4 CUTANA CUT RUN Antibody抗體

參  考  價(jià):5643
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  • 型號(hào)

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    EpiCypher

  • 廠商性質(zhì)

    代理商

  • 所在地

    深圳市

規(guī)格

50 µL 5643元 59件可售

更新時(shí)間:2024-05-22 15:02:48瀏覽次數(shù):433次

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供貨周期 兩周 規(guī)格 50 μL
貨號(hào) 13-2003 應(yīng)用領(lǐng)域 生物產(chǎn)業(yè),綜合
主要用途 科研
EpiCypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。EpiCypher推出的CUT&RUN級(jí)別的BRD4 Antibody符合EpiCypher的“CUTANA Compatible"標(biāo)準(zhǔn),可用于核酸酶靶向切割和釋放(CUT&RUN)和/或靶向剪切及轉(zhuǎn)座酶技術(shù)(CUT&Tag)的基因組定位。

EpiCypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。EpiCypher推出的CUT&RUN級(jí)別的BRD4 Antibody符合EpiCypher的“CUTANA Compatible"標(biāo)準(zhǔn),可用于核酸酶靶向切割和釋放(CUT&RUN)和/或靶向剪切及轉(zhuǎn)座酶技術(shù)(CUT&Tag)的基因組定位。BRD4抗體主要在轉(zhuǎn)錄起始位點(diǎn)的側(cè)翼產(chǎn)生CUT&RUN峰(Fig. 1)。正如文獻(xiàn)所報(bào)道,BRD4峰與BRG1/SMARCA4峰有很大程度的重疊(Fig. 2)。


產(chǎn)品詳情

反應(yīng)種屬: Human, Mouse

宿主來(lái)源: Rabbit

實(shí)驗(yàn)應(yīng)用: CUT&RUN, IHC, IP, WB

免疫原: Between amino acids 1312 and 1362

克隆性: Polyclonal

保存溫度: Stable for 1 year at 4°C from date of receipt

運(yùn)輸溫度: DO NOT FREEZE!! Frozen cold packs.

產(chǎn)品形式: Antigen affinity-purified antibody in Tris-citrate/phosphate buffer pH 7-8, 0.09% sodium azide


驗(yàn)證數(shù)據(jù)

BRD4 CUTANA CUT RUN Antibody抗體


Figure 1: BRD4 peaks in CUT&RUN

CUT&RUN was performed as described above. Peaks were called with MACS2. Heatmaps show BRD4 peaks relative to IgG negative control antibody and H3K4me3 positive control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. All rows were aligned relative to H3K4me3 antibody.

BRD4 CUTANA CUT RUN Antibody抗體



Figure 2: BRD4 CUT&RUN representative browser tracks

CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci show overlap of BRD4 and BRG1 peaks.

BRD4 CUTANA CUT RUN Antibody抗體

Figure 3: Immunohistochemistry data

FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) using BRD4 antibody at a dilution of 1:5,000.



BRD4 CUTANA CUT RUN Antibody抗體


Figure 4: Immunoprecipitation data

EpiCypher BRD4 antibody (6 µg) was used to immunoprecipitate whole cell lysates (1 mg, 20% of IP loaded) isolated from HeLa cells. A negative control IgG antibody and positive control antibody targeting BRD4 (Bethyl Laboratories) were also used to demonstrate the specificity of the IP. For blotting immunoprecipitates, Bethyl Laboratories BRD4 antibody (A700-004) was used at a dilution of 1:1,000.


訂購(gòu)詳情

貨號(hào)

產(chǎn)品名稱

規(guī)格

13-2003

BRD4 CUTANA™ CUT&RUN Antibody

50 µL




如需了解更多詳細(xì)信息或相關(guān)產(chǎn)品,請(qǐng)聯(lián)系EpiCypher中國(guó)授權(quán)代理商-欣博盛生物



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