Recombinant Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14)

肿瘤坏死因子受体超家族成员14(TNFRSF14)重组蛋白

[ PROPERTIES ]

Residues: Ser41~Ser209,with two N-terminal Tags, His-tag and T7-tag.

Host:E. coli

Subcellular Location: Membrane; Single-passtype I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 21.8kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8°C  for one month.

           Aliquot and store at -80°C  for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SCKEDEYPVGSECCPKCSPGYRVKEACGEL TGTVCEPCPP GTYIAHLNGL SKCLQCQMCD PAMGLRASRN CSRTENAVCGCS P G H F CI VQ DG D H CA A CRA YATS S PG Q RV QK G G T ES Q D T LC Q N CPP G T FSPNGTLEECQ HQTKCSWLVT KAGAGTSSSH WVWWFLSGS

[  IDENTIFICATION  ]

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