二胺氧化酶(DAO)檢測試劑盒

適用生物 Homo sapiens (Human，人)
二胺氧化酶(DAO)檢測試劑盒檢測范圍 0.313-20ng/mL 靈敏度 0.135ng/mL
樣本類型 Serum, plasma, tissue homogenates and other biological fluids.
實(shí)驗(yàn)時(shí)長 4.5h 實(shí)驗(yàn)方法 雙抗夾心法 二胺氧化酶(DAO)檢測試劑盒規(guī)格 96T

ELISA Kit for Diamine Oxidase (DAO)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

二胺氧化酶(DAO)檢測試劑盒Specificity

This assay has high sensitivity and excellent specificity for detection of Diamine Oxidase (DAO).
No significant cross-reactivity or interference between Diamine Oxidase (DAO) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Diamine Oxidase (DAO) and the recovery rates were calculated by comparing the measured value to the expected amount of Diamine Oxidase (DAO) in samples.

二胺氧化酶(DAO)檢測試劑盒Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diamine Oxidase (DAO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diamine Oxidase (DAO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Diamine Oxidase (DAO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

二胺氧化酶(DAO)檢測試劑盒Reagents and materials provided

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37^oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37^oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37^oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37^oC;
8. Add 50μL Stop Solution. Read at 450nm immediay.

二胺氧化酶(DAO)檢測試劑盒Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diamine Oxidase (DAO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diamine Oxidase (DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diamine Oxidase (DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diamine Oxidase (DAO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.