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膠原蛋白I型說明書

閱讀:1924      發(fā)布時(shí)間:2015-4-7
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Collagen from bovine achilles tendon 膠原蛋白I型說明書 
Product Number: C8060
CAS Number : 9007-34-5
Storage Temperature: 2-8°C
Product Description
This product is suitable for use as a substrate for collagenase. It is prepared by modification of the method of Einbinder
and Schubert and is not suitable for use in coating glassware.
This collagen is Bornstein and Traub Type I. The Type I classification of collagen is a component of skin, bone, tendon,
and other fibrous connective tissues. Type I collagen differs from other collagens by their low lysine hydroxylation
and low carbohydrate composition.
Collagen breaks down metabolically in the body to release N-opeptide, which is the N-terminus of collagen. There
is also C-opeptide, which is presumably the C-terminus. N-opeptide is released in urine, and its detection in
diagnostic tests is used to screen for osteoporosis.
Although different types of collagen exist, they are all composed of molecules containing three polypeptide chains
arranged in a triple helical conformation. Slight differences in the primary structure (amino acid sequence) establish
differences between the types. The amino acid sequence of the primary structure is mainly a repeating motif with
glycine in every third position and proline or 4-hydroxyproline frequently preceeding the glycine residue.
Preparation Instructions
This product is an insoluble collagen preparation. It is insoluble in water, aqueous buffers, dilute acid, and organic
solvents. For use as a substrate in collagenase assays, this collagen can be prepared as a suspension in 50 mM TES
buffer, pH 7.4 with 0.36 mM calcium chloride. but not a clear solution.
Storage/Stability
It is recommended to store this product at 2–8 °C. If stored as recommended, it will have a shelf-life of up to 8 years.
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.

Solarbio
Beijing Solarbio Science & Technology Co., Ltd


Http://www.solarbio-hd。。cn


膠原蛋白I型說明書

產(chǎn)品數(shù)量:C8060

CAS編號:9007-34-5

存儲(chǔ)溫度:2 - 8°C

產(chǎn)品描述

本產(chǎn)品適用于使用作為膠原酶的底物。它是由Einbinder的修改方法和舒伯特,并不適用于鍍膜玻璃器皿。這個(gè)膠原蛋白是波恩和特勞布類型一、I型膠原蛋白是皮膚的一個(gè)組成部分,分類骨骼,肌腱,

和其他纖維結(jié)締組織。I型膠原蛋白不同于其他膠原蛋白低賴氨酸羥基化和低碳水化合物組成。

膠原蛋白在體內(nèi)分解代謝釋放N-opeptide,膠原蛋白分子的n端。有也是C-opeptide,想必C-terminus。尿液N-opeptide釋放,其檢測診斷測試篩選用于骨質(zhì)疏松癥。

盡管不同類型的膠原蛋白存在,他們都包含三個(gè)多肽鏈組成的分子

安排在一個(gè)三重螺旋構(gòu)象。輕微的差異(氨基酸序列)建立的主要結(jié)構(gòu)

類型之間的差異。的氨基酸序列一級結(jié)構(gòu)主要是一個(gè)重復(fù)的主題

在每個(gè)第三位置和脯氨酸或甘氨酸4-hydroxyproline經(jīng)常在甘氨酸殘基前夕。

本產(chǎn)品是一種不溶性膠原蛋白制備。它不溶于水,水溶液緩沖區(qū),稀酸和有機(jī)的

溶劑。作為基質(zhì)的膠原酶化驗(yàn),這種膠原蛋白可以作為懸掛在50 mM的te做好準(zhǔn)備

緩沖區(qū),pH值7.4與0.36毫米氯化鈣。但不是一個(gè)明確的解決方案。

存儲(chǔ)/穩(wěn)定

建議儲(chǔ)存該產(chǎn)品在2 - 8°C。如果存儲(chǔ)為建議,它將有8年的保質(zhì)期。

預(yù)防措施和免責(zé)聲明

僅供實(shí)驗(yàn)室使用。不是藥物,家庭或其他用途。


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