Mouse GCGR CRE-Luc HEK293
| I. Background | |
胰高血糖素是一种胰腺肽激素，作为胰岛素的反调节激素，刺激葡萄糖通过肝脏释放， 维持葡萄糖稳态。GCGR 是一种 B 类 GPCR，最初被描述为与腺苷酸环化酶功能相连的胰 高血糖素结合体。它在维持葡萄糖稳态中起关键作用，因此被认为是治疗糖尿病的一个有 价值的靶点。 | |
| II. Description | |
Mouse GCGR CRE-Luc HEK293 报告基因药靶模型很好的模拟了体内 GCGR 的信号转导过程，原理见下图所示。
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| III. Introduction | |
| Expressed gene: | Mouse GCGR |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+200 μg/ml Hygromycin B+2 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional assay for Mouse GCGR |
| Transducer: | Gs |
IV. Representative Data
Figure 2. Dose Response of Glucagon(1-29) in Mouse GCGR CRE-Luc HEK293(C17). | |
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