hGUCY2C/4T1 hGUCY2C/4T1 hGUCY2C/4T1
| I. Background | |||
| 鳥苷酸環(huán)化酶 C（guanylyl cyclase C， GUCY2C/GCC）屬于受體鳥苷酸環(huán)化酶家族中 的一員，該蛋白可作為內(nèi)源性肽鳥苷蛋白和尿鳥苷蛋白以及耐熱大腸桿菌腸毒素的受體。 GUCY2C 信號傳導由內(nèi)源激素鳥苷蛋白和尿鳥苷蛋白（分別為 GUCA2A 和 GUCA2B）和 外源細菌熱穩(wěn)定腸毒素 ST 誘導，產(chǎn)生第二信使 cGMP，激活 cGMP 依賴性蛋白酶 （PKG），最終導致上皮細胞增殖、分化和細胞凋亡等腸道穩(wěn)態(tài)過程的調(diào)節(jié)。GUCY2C 廣 泛表達于結直腸癌細胞和其他胃腸道腫瘤，GUCY2C 的功能喪失或增加與便秘或腹瀉相關 的幾種疾病有關，包括腸易激綜合征伴便秘（IBS-C）和慢性特發(fā)性便秘（CIC）以及炎癥 性腸病（IBD）。 | |||
| II. Introduction | |||
| Host Cell: | 4T1 | ||
| Expressed gene: | hGUCY2C | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+2μg/ml puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mouse | ||
| Tissue: | Mammary gland | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | |||
Figure 1. Recombinant hGUCY2C/4T1 stably expressing hGUCY2C. | |||
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