TIE2/HEK293
| I. Background | |
| TIE2（激酶受体）主要表达于血管内皮细胞表面的受体，胞外区含免疫球蛋白 域 （ 结 合 ANG ） 、 EGF 样 域 （ 介 导 二 聚 化 ） ； 胞内区含激酶活性位点 （Y1100/Y1108）。被配体激活后通过 PI3K/AKT 和 MAPK 通路调控内皮细胞存活、迁移 及血管屏障功能。在病理状态下（如脓毒症、肿瘤），TIE2 信号异常导致血管渗漏或异常 增生。 | |
| II. Description | |
| TIE2 HEK293 细胞，在 HEK293 细胞的基础上，稳定表达人 TIE2。 | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | TIE2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | TIE2,Tie-2,TEK,VMCM, VMCM1,CD202b,Angiopoietin-1 receptor ... |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10% FBS+2 μg/ml Puromycin |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 1. Recombinant HEK293 cell constitutively expressing TIE2. | |
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