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供貨周期	現(xiàn)貨	規(guī)格	T-25 Flask
貨號(hào)	CBP74030	應(yīng)用領(lǐng)域	生物產(chǎn)業(yè)
主要用途	僅限科研使用

CBP74030
I. Background
The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.

II. Description
The SRE Reporter -HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter -HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.

III. Introduction
Host Cell:	HEK293
Expressed gene:	SRE-Luciferase
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	SRE cell line, MAPK reporter, ERK reporter
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	DMEM+10%FBS+200ug/ml hygromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Embryonic kidney
Disease:	Normal
Morphology:	Epitheloid cell
Growth Properties:	Adherent

Ⅴ. Representative Data

Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human EGF in SRE/Luc HEK293 Cells (C5C16).