| I. Background | |
糖皮質(zhì)激素誘導(dǎo)的腫瘤壞死因子受體相關(guān)蛋白（GITR，也稱為 TNFRSF18 或 CD357），屬于腫瘤壞死因子受體超家族（TNFRSF），它在多種免疫細(xì)胞上表達，包括 效應(yīng) T 細(xì)胞和調(diào)節(jié)性 T 細(xì)胞。GITR 在調(diào)節(jié)免疫系統(tǒng)中發(fā)揮重要作用，尤其是在 T 細(xì)胞的 活化和調(diào)節(jié)性 T 細(xì)胞（Treg）的功能中。 | |
| II. Description | |
| GITR NFκB-Luc Jurkat 報告基因藥靶模型很好的模擬了體內(nèi) GITR 的信號轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。
Figure 1. GITR NFκB-Luc Jurkat 細(xì)胞模型原理圖 | |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | GITR-NFκB-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 μg/ml Puromycin+400 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Peripheral blood |
| Disease: | Childhood T acute lymphoblastic leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 2. Recombinant Jurkat stably expressing human GITR.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human TNFSF18 in GITR-NFκB-Luc Jurkat(C1) Dose Response of GITR NFκB-Luc Jurkat(C1) Stimulated by GITRL CHO (C1).
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