| I. Background | |
| APOE 是一种载体，参与脑内脂质运输和修复。APOE 是一种多态蛋白，具有 APOE4、APOE3 和 APOE2 三种异构体，它们之间存在两个氨基酸替换（分别为 Arg/Arg、 Cys/Arg 和 Cys/Cys），导致三级结构不同，并可能改变 APOE 的功能。尽管异构体之间存 在相似性，但它们在发生 AD 的风险方面存在重大差异，其中 APOE4 是 AD 发生的强烈危 险因素。APOE3 是最常见的亚型，不影响患 AD 的风险，而 APOE2 与患 AD 的风险降低有关。 | |
| II. Description | |
APOE TCR Activator CHO 作为 LILRB4 Effector Reporter Cell 的靶细胞，很好的模拟了 体内 LILRB4 的信号转导过程，原理见下图所示。 Figure 1. APOE TCR Activator CHO 模型原理图 | |
| III. Introduction | |
| Host Cell: | CHO |
| Expressed gene: | APOE |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Apolipoprotein E |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+600μg/ml hygromycin+2μg/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Cricetulus griseus, hamster, Chinese |
| Tissue: | Ovary |
| Disease: | Hamster Chinese ovary |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| V. Representative Data | |
Figure 2. Recombinant APOE/TCR Activator/CHO constitutively expressing APOE. | |
Figure 3. Dose response of LILRB4 Blocking antibody in LILRB4 Effector Reporter Cell(C5) With APOE TCR Activitor CHO. | |
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