| CBP73269 | |
| I. Introduction | |
Cell Line Name： | c-Kit WT (SCF1 Dependent)/BaF3 |
Host Cell: | BA/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS+0.25ug/ml Recombinant Human SCF Protein (R&D) |
Mycoplasma Status: | Negative |
| II.Background | |
Human c-Kit also known as KIT, PBT, CD117, Piebald Trait, SCFR, and Mast/Stem Cell Growth Factor Receptor, GenBank Accession No. NM_000222, a.a. 544-976 with N-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa. | |
| III. Representative Data | |
1. WB of c-Kit WT (SCF1 Dependent)/BaF3
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2. Anti-proliferation assay
Figure 2. CTG Proliferation Assay of BaF3 c-KIT BaF3 Cells(C1).. | |
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