RM-1-Luc示踪细胞株 CBP30175L | |
| I. Introduction | |
| Host Cell: | RM-1 |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+5ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | Mouse prostate cancer primary site: Subcapsular renal graft. Ras+Myc-induced prostate cancer that developed from a urogenital sinus mouse prostate reconstitution. |
| Disease: | Prostate cancer, mouse |
| Morphology: | Fibroblast-like |
| Growth Properties: | Adherent |
| III. Representative Data | |
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Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.. The number of Luminescence signal is 518±16(Mean±SD) on RM-1 cells while 216452±9462(Mean±SD) on RM-1-Luc cells. | |
| IV. Stablity Test | |
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Figure. Stablity test results.RM-1-Luc cells showed at least 32 passage stability with similar luminescence unit. | |
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