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SMPH,SMPB,SMCC,SPDP,SIA交聯(lián)劑

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SMPH,SMPB,SMCC,SPDP,SIA交聯(lián)劑 SPDP交聯(lián)劑是可分解的,不溶于水,氨和硫醇(巰基)反應heterobi功能蛋白交聯(lián)劑。SPDP交聯(lián)試劑的反應的moites由N-羥基琥珀酰亞胺(NHS)酯與伯胺反應。在另一端的pyridinyldisulfide SPDP,反應sulhydryls產(chǎn)生可逆的二硫鍵。

SMPH,SMPB,SMCC,SPDP,SIA交聯(lián)劑

SMPH,SMPB,SMCC,SPDP,SIA交聯(lián)劑

 

產(chǎn)品名稱:SIA

英文名稱:N-Succinimidyl Iodoacetate

中文名稱:碘乙酸 N-羥基琥珀酰亞胺酯

CAS #: 39028-27-8

簡介:N-Succinimidyl iodoacetate (SIA) is a non-cleavable, heterobifunctional protein crosslinker. SIA is among the shortest amine and sulfhydryl (thiol) reactive crosslinkers known with a spacer arm length of 1.5 Angstroms.

具有氨基和巰基反應性的雙異官能團交聯(lián)劑。情況下,初通過 pH 7.5 (6.5-8.5) 緩沖條件下的酰胺鍵偶聯(lián)到含有伯胺的分子上,接著通過 pH 7.5 (7.0-8.0) 緩沖條件下的硫醚鏈與含有自由巰基的分子進行特異性次級鍵合。用于制備交聯(lián)劑和半抗原載體分子偶聯(lián)物。提供兩個原子的連接基。

外觀:白色粉末

Molecular Weight: 283.02

分子式:C6H6INO4

溶解性:DMF

CAS #: 39028-27-8

Spacer Length: 1.5 Å

Alternative Names: Iodoacetic acid N-hydroxysuccinimide ester; Succinimidyl iodoacetate

純度:98%+

保存:-20

:西安瑞禧生物科技有限公司

 

 

產(chǎn)品名稱:SMCC

英文名:N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate

中文名:4-(N-馬來酰亞胺甲基)環(huán)己烷羧酸-N-琥珀酰亞胺酯

CAS #: 64987-85-5

簡介:SMCC crosslinker (Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate) is a heterobi-functional protein crosslinker with a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive. The SMCC crosslinking reagent is permeable across the lipid bilayer of the cell membrane. The 9 atom spacer arm of SMCC is not cleavable.

SMCC crosslinkersuccinimidyl-4 - [ n-maleimidomethyl ] cyclohexane-1-carboxylate)是一個heterobi功能蛋白的交聯(lián)劑與馬來酰亞胺組是巰基(硫醇;SH)活性和NHS酯組胺反應。市建委交聯(lián)劑在膜的脂質(zhì)雙層膜的滲透。點9原子間隔臂是不可。

Application

4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester (SMCC) is useful for the preparation of enzyme immunoconjugates and hapten carrier molecule conjugates. It has been used for the coupling of a peptide sequence to an amino group

 

外觀:白色粉末

分子式:C16H18N2O6

Molecular Weight: 334.3

CAS #: 64987-85-5

溶解度:DMF

Spacer Length: 8.3 Å

Alternative Names: 4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester;

純度:98%+

保存:-20

:西安瑞禧生物科技有限公司

 

 

產(chǎn)品名稱:SMPB

英文名稱:N-Succinimidyl 4-(p-Maleimidophenyl)butyrate

文名稱:4-(p-馬來酰亞胺苯基)丁酸琥珀酰亞胺酯

CAS #: 79886-55-8

簡介:SMPB crosslinker (N-Succinimidyl 4-[4-maleimidophenyl]butyrate) is a heterobifunctional pro-tein crosslinking reagent that is reactive toward sulfhydryl and amino groups. The SMPB pro-tein crosslinker is an extended chain length analog of MBS crosslinker.

SMPB crosslinker4 - [其他]馬來酰亞胺基苯丁酸)是一種雙功能蛋白交聯(lián)試劑,反應對巰基和氨基的基團。該市人事局蛋白交聯(lián)劑是一種擴展鏈長度MBS交聯(lián)劑。

Molecular Weight: 356.3

分子式:C18H16N2O6

CAS #: 79886-55-8

Spacer Length: 11.6 Å

Alternative Name: 4-(4-Maleimidophenyl)butyric acid N-hydroxysuccinimide ester

純度:98%+

保存:-20

:西安瑞禧生物科技有限公司

 

產(chǎn)品名稱:SMPH

英文名稱:(succinimidyl-6-((b-maleimidopropionamido)hexanoate)

簡介:SMPH crosslinker (Succinimidyl-6-[ß-maleimidopropionamido]hexanoate) is a water insoluble, heterobifunctional protein crosslinker with a non-cleavable spacer arm length of 14.3 angstroms. The SMPH crosslinking reagent has maleimide groups which are reactive to thiols (sufhydryls) and its NHS ester groups react with amines at pH 6.5-7.5 and 7-9, respectively

SMPH crosslinkersuccinimidyl-6 - [ß- maleimidopropionamido ]己酸)是一種不溶于水,具有不可切割的間隔臂的長度為14.3埃的異型蛋白交聯(lián)劑。植株的交聯(lián)劑具有活性巰基馬來酰亞胺基團(sufhydryls)和NHS酯基團反應在pH 6.5-7.5,7-9

General description

SMPH is a heterobifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while the maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values greater than 7.5. For these reasons, conjugation experiments involving this type of heterobifunctional crosslinker are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

 

結(jié)構式:

 

外觀:白色粉末

Molecular Weight: 379.4

分子式:C17H21N3O7

溶解:DMSO or DMF

Alternative Name: Succinimidyl-6-[βmaleimidopropionamido] hexanoate

純度:98%+

保存:-20

:西安瑞禧生物科技有限公司

 

 

產(chǎn)品名稱:SPDP

英文名稱:N-Succinimidyl 3-(2-Pyridyldithio)propionate

中文名稱:3-(2-吡啶基二硫基)丙酸N-羥基琥珀酰亞胺酯

CAS #: 68181-17-9

簡介:SPDP crosslinker is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive heterobi-functional protein crosslinker. The SPDP crosslinking reagent's reactive moites consist of an N-hydroxysuccinimide (NHS) ester that reacts with primary amines. On the other end of SPDP, the pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

SPDP交聯(lián)劑是可分解的,不溶于水,氨和硫醇(巰基)反應heterobi功能蛋白交聯(lián)劑。SPDP交聯(lián)試劑的反應的moitesN-羥基琥珀酰亞胺(NHS)酯與伯胺反應。在另一端的pyridinyldisulfide SPDP,反應sulhydryls產(chǎn)生可逆的二硫鍵。

Application

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is useful for the preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.

It has been used for antibody conjugation and as a protein cross-linker.

 

General description

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is a heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. It is typically coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternay, this reagent may be used as a protected thiolating reagent following initial coupling and reduction of linker. It incorporates four atom linker.

 

外觀:白色粉末

Molecular Weight: 312.4

分子式:C12H12N2O4S2

CAS #: 68181-17-9

溶解:DMSO or DMF

Spacer Length: 6.8 Å

Alternative Name: 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester

純度:98%+

保存:-20

:西安瑞禧生物科技有限公司

 

 

產(chǎn)品名稱:Sulfo-LC-SPDP

英文名稱:(sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate)

簡介:Sulfo-LC-SPDP crosslinking reagent is a cleavable, water soluble, amino and thiol (sulfhydryl) reactive heterobifunctional protein crosslinker.

Sulfo-LC-SPDP crosslinker's reactive moites consist of an N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines. The other end of the Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

LC SPDP交聯(lián)試劑磺可裂解、水溶性氨基和巰基反應異型雙功能交聯(lián)劑(巰基)蛋白。

SPDP交聯(lián)劑的反應moitesLC組成的N-羥基(磺基NHS)酯與伯胺反應。的LC SPDP交聯(lián)分子pyridinyldisulfide磺化反應sulhydryls產(chǎn)生可逆的二硫鍵的另一端。

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

General description

The SPDP reagents are a unique group of amine- and sulfhydryl-reactive heterobifunctional crosslinkers. Whether they are used to form amine-to-amine or amine-to-sulfhydryl crosslinks among molecules, the SPDP reagents produce disulfide-containing linkages that can be cleaved later with reducing agents such as dithiothreitol (DTT).

 

外觀:白色粉末

Molecular Weight: 527.6

分子式:C14H16N3NaO8S3

溶解:水中

Spacer Length: 15.7 Å

Alternative Name: Sulfosuccinimidyl-6-(3’-[2-pyridyldithio]-propionamido) hexanoate

純度:98%+

保存:-20

:西安瑞禧生物科技有限公司

 

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