膜染料 DAPI Ex/Em (nm) 358/461
膜染料 DAPI Ex/Em (nm) 358/461
膜染料 DAPI Ex/Em (nm) 358/461
膜染料 DAPI Ex/Em (nm) 358/461
Overview | Printer Friendly Version |
Ex/Em (nm) | 358/461 |
MW | 350.25 |
CAS # | 28718-90-3 |
Solvent | Water |
Storage | F/D/L |
Category | Nucleic Acid Detection DNA Detection |
Related | Labeling Cells Fluorescence Imaging Biochemical Assays |
DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.
西安瑞禧生物科技有限公司是的荧光染料,我们提供的荧光染料的吸收波长和发射波长从300nm-810nm不等,我们可以再不同的荧光染料上面做的活性基团,比如NHS、amine、COOH、azide、alkyne、maleimide等等。关于荧光染料我们可以提供FITC、Rhodamine、Bodipy系列染料、Coumairn 染料、Dansyl染料、FAM/5-TAMRA、NBD、CY系列、ICG及IR系列、ATTO系列染料、AMCA、Perylene、碘化物、DIR/DIL/DIO/DID/DAPI膜染料等等。我公司特别在近红外荧光染料Near infrared dyes方面有的产品线。
Bodipy系列染料(氟化硼二吡咯类荧光染料)
Bodipy氟化硼二吡咯类荧光染料相对比于荧光素、罗丹明或者菁染料有着较的量子产率、摩尔消光系数、的光学稳定性和对PH不的被应用于生物标记、荧光探针及生物成像,西安瑞禧生物科技有限公司可以提供的Bodipy荧光染料。
瑞禧生物的BODIPY类荧光染料衍生物:
1:比50%
2:包装量比大一倍
3:95%+
4:可以提供相关检测图谱及
5:如有质量问题
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