QQ交談
產(chǎn)品展示
Restriction EnzySigma-Aldrich Cloning Expres
- 公司名稱：
- 更新時間：
- 所 在 地：
- 生產(chǎn)地址：
- 瀏覽次數(shù)：
- 上海輔澤商貿(mào)有限公司
- 2018-08-17 15:32:00
- 上海市
- 323
【簡單介紹】
| 品牌 | Sigma-Aldrich | 貨號 | Acc I (R6142) |
|---|---|---|---|
| 供貨周期 | 一周 |
【詳細(xì)說明】
Restriction Enzyme Buffer Reference
Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer for each enzyme (table 2).
Table 1
Activity (%) of each Sigma restriction enzyme in each Sigma buffer. The preferred buffer for each enzyme is highlighted in bold.
| Buffer | |||||
| Restriction Enzyme | SA (B7531) | SB (B8781) | SL (B3782) | SM (B3158) | SH (B3657) |
| Acc I (R6142) | 100 | 0-10 | 10-25 | 1-10 | 0-10 |
| Alu I (R6885) | 100 | 50-75 | 25-50 | 25-50 | 0-10 |
| Apa I (R4258) | 100 | 10-25 | 50-75 | 50-75 | 0-10 |
| Bam HI (R0260) | 100 | 100 | 75-100 | 100 | 25-50 |
| Bcl I (R8631) | 100 | 100 | 25-50 | 100 | 100 |
| Bgl II (R6377) | 100 | 100 | 25-50 | 100 | 100 |
| Bln I (R3131) | 25-50 | 50-75 | 0-10 | 25-50 | 100 |
| Bsm I (R3635) | 0-10 | 50-75 | 0-10 | 25-50 | 100 |
| Cfo I (R1761) | 75-100 | 50-75 | 100 | 50-75 | 25-50 |
| Cla I (R7763) | 100 | 100 | 75-100 | 100 | 100 |
| Dde I (R4256) | 50-75 | 75-100 | 25-50 | 25-50 | 100 |
| Dpn I (R8381) | 100 | 75-100 | 50-75 | 75-100 | 75-100 |
| Dra I (R4381) | 100 | 75-100 | 100 | 100 | 50-75 |
| Ecl XI (R3884) | 25-50 | 100 | 25-50 | 25-50 | 50-75 |
| Eco RI (R6265) | 100 | 100 | 25-50 | 50-75 | 100 |
| Eco RV (R2756) | 25-50 | 100 | 0-10 | 25-50 | 50-75 |
| Hae III (R5628) | 50-75 | 50-75 | 75-100 | 100 | 25-50 |
| Hind III (R1137) | 50-75 | 100 | 25-50 | 100 | 50-75 |
| Hpa I (R8507) | 100 | 25-50 | 25-50 | 50-75 | 25-50 |
| Kpn I (R1258) | 75-100 | 10-25 | 100 | 25-50 | 0-10 |
| Nco I (R8761) | 50-75 | 50-75 | 50-75 | 50-75 | 100 |
| Nde I (R5509) | 25-50 | 100 | 10-25 | 50-75 | 100 |
| Nhe I (R5634) | 100 | 25-50 | 100 | 100 | 10-25 |
| Not I (R8506) | 10-25 | 50-75 | 0-10 | 25-50 | 100 |
| Nsi I (R5884) | 50-75 | 100 | 10-25 | 50-75 | 100 |
| Pst I (R7023) | 25-50 | 25-50 | 10-25 | 25-50 | 100 |
| Pvu I (R1508) | 50-75 | 75-100 | 25-50 | 50-75 | 100 |
| Pvu II (R2631) | 25-50 | 25-50 | 25-50 | 100 | 25-50 |
| Rsa I (R4756) | 100 | 50-75 | 100 | 50-75 | 0-10 |
| Sac I (R5268) | 100 | 0-10 | 100 | 50-75 | 0-10 |
| Sal I (R0754) | 0-10 | 25-50 | 0-10 | 10-25 | 100 |
| Sau 3A (R0762) | 100 | 25-50 | 25-50 | 75-100 | 0-10 |
| Sca I (R5007) | 0-10 | 100 | 0-10 | 75-100 | 100 |
| Sfi I (R8256) | 25-50 | 25-50 | 75-100 | 100 | 25-50 |
| Sma I (R4503) | 100 | 0-10 | 0-10 | 0-10 | 0-10 |
| Spe I (R5257) | 75-100 | 75-100 | 75-100 | 100 | 100 |
| Sph I (R7135) | 50-75 | 75-100 | 25-50 | 100 | 75-100 |
| Ssp I (R6759) | 75-100 | 75-100 | 10-25 | 75-100 | 100 |
| Taq I (R9507) | 50-75 | 100 | 25-50 | 50-75 | 50-75 |
| Xba I (R7260) | 100 | 75-100 | 75-100 | 75-100 | 100 |
| Xho I (R6759) | 25-50 | 75-100 | 10-25 | 25-50 | 100 |
Table 2
The complete formulation of each Sigma Restriction Enzyme Buffer. The formulation of the storage buffer for each Sigma restriction enzyme is given on the catalog page (see links in table 1).
| Final concentration (mM) | |||||
| SA (B7531) | SB (B8781) | SL (B3782) | SM (B3158) | SH (B3657) | |
| Tris-acetate | 33 | ||||
| Tris-HCl | 10 | 10 | 10 | 50 | |
| Mg-acetate | 10 | ||||
| MgCl2 | 5 | 10 | 10 | 10 | |
| K-acetate | 66 | ||||
| NaCl | 100 | 50 | 100 | ||
| Dithioerythritol (DTE) | 1 | 1 | 1 | ||
| Dithiothreitol (DTT) | 0.5 | ||||
| 2-Mercaptoethanol (BME) | 1 | ||||
| pH at 37°C | 7.9 | 8.0 | 7.5 | 7.5 | 7.5 |
相關(guān)產(chǎn)品
- MXA22168尼康鏡油 30CC
- IL-21 NK細(xì)胞擴(kuò)增試劑/NK細(xì)胞無血清培養(yǎng)基
- 73245Stains/Dyes
- SRP4754Signaling Molecule （Marker）
- T8694Receptors
- LYSISO1Organelle, Membrane-specific Kit
- M9195Dissociation
- SRP3190Growth Factors
- B0814Cytokines
- SRP3191Chemokines
- S1694Stemline®
- 51800CSpecialty Media
- R8758Classical Media
- SRP3196Vitamins, Hormones, Misc.
- SRP4595Signaling molecules
- A4941Proteins Peptides
- C3742Inhibitors
- BPQ24Bioactives/Supplements Kits
- C4428Bioactives/Supplements Enzymes
- A5228Antibodies against Proteins, Bioactives
- B9553Antibodies against Cytokines, Chemokines
- C2542Antibodies against Adhesion, Attachment
- M9195Inter / Intra Cellular Proteins, Enzymes
- H4777Extracellular Matrix Components
- S1694Stem Cell Culture Media
- DNAQFStem Cell Markers
- T8694Receptors for Stem Cell Biology
- NXTRACTOrganelle/Membrane-specific
- N7534Multiple Tissue Northern Blots
- A5691Antibodies against Proteins/Bioactives
- N4763General Stains/Dyes for Stem Cell
- SRP3296Signaling Molecule （Marker）
- DMN10RNA/DNA Isolation Products for Stem
- SHC001Stem Cell Transfection/Transduction
- C2542Antibodies against Adhesion/Attachment
- P8115核酸擴(kuò)增
- SRP3181Bioactives/Supplements
- H0268Expression/Translation
- SRP3015Cytokines, Growth Factors, Chemokines
- I5270Antibodies against Cytokines/Growth
產(chǎn)品搜索
請輸入產(chǎn)品關(guān)鍵字：
聯(lián)系方式
地址：上海市虹口區(qū)曲陽路910號復(fù)城國際1101
郵編：200437
聯(lián)系人：鄭經(jīng)理
電話：13816982406,021-65010900
傳真：021-65010900
手機(jī)：13816982406
留言：發(fā)送留言
網(wǎng)址：
商鋪：http://www.cylcaiwu.com/st215208/
郵編：200437
聯(lián)系人：鄭經(jīng)理
電話：13816982406,021-65010900
傳真：021-65010900
手機(jī)：13816982406
留言：發(fā)送留言
網(wǎng)址：
商鋪：http://www.cylcaiwu.com/st215208/