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Emfret M023-2说明书
Cat.No.:XX | M023-2 |
抗原： | 整合素αIIbbeta3（GPIIb / IIIa，CD41 / CD61） |
描述： | PE缀合的JON / A抗体选择性结合小鼠整联蛋白αIIbβ3的高亲和力构象，小鼠整合素αIIbβ3是许多配体的血小板/巨核细胞特异性受体，包括血管性血友病因子（vWf），纤维蛋白原，纤维连接蛋白和玻连蛋白。 。 |
克?。?/span> | JON / A. |
同种型： | 大鼠IgG2b |
形成： | PE |
应用： | FC（活动表格） |
尺寸： | 1.5毫升; 300次测试 |
价钱： | 499.00美元 |
特异性：PE结合的jon/A抗体选择性地结合小鼠整合素aIIb 3 1(GPIIbIIIa，CD 41/CD 61)的高亲和力构象，这是一种由135 kDa ai组成的糖蛋白复合物。 IB链和90 kDa b3链。整合素aIIbb3是纤维蛋白原、血管性血友病因子、纤维连接蛋白和维甲酸的血小板受体，介导血小板粘附和聚集。A型 整合素aIIbb3中的阳离子依赖性构象变化，因此乔恩/A-PE的结合依赖于细胞外游离钙。
制备和保存：用蛋白G-Sepharose层析法从杂交瘤细胞培养上清液中纯化抗体.该抗体与R-生理红蛋白(PE)在宜条件下结合。 尼迪奇。将产品保存在4°C未稀释，避免长时间暴露在光线下。从装运之日起稳定一年。别冻着。
用途：本抗体制备方法适用于流式细胞术应用：推荐用5μl染色~106血小板或~0.5x 106细胞，体积为25μl泰氏-Hepes缓冲液。 Ng 1毫米CaCl 2室温下孵育15 min，加入400μmol/L的PBS停止反应，30 min内对样品进行分析。请注意孵化时间、缓冲条件的变化。 S，或抗体浓度可能影响琼恩/A-PE的结合.
警告： Sodium azide是一种可逆的氧化代谢抑制剂；因此，含有这种防腐剂的抗体制剂不得用于细胞培养或动物体内注射。 。Sodium azide可以通过洗涤染色细胞或平板结合抗体或在无Sodium azide缓冲液中透析可溶性抗体来去除。
emfret授权书：
EMFRET Analytics—专注脉管系统抗体
德国EMFRET公司专注心血管和血液系统研究用抗体研发和生产，供应的抗体包括清除血液中血小板、流式细胞术鉴定分析小鼠血小板表面糖蛋白，EMFRET公司的抗体不仅适用于流失分析、WB检测，还可用于免疫组化/免疫荧光及免疫共沉淀检测。
Cat.No. | Antigen | Clone | Isotype | Form | Applications | Size |
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C301 | Antibodies for Mouse Plaet Depletion | polyclonal non-immune rat immunoglobulins | Rat IgG | purif. | negative control for #R300 | 0.5 mg |
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D200 | Two-Color Analysis of Mouse Plaet Activation | Wug.E9 JON/A | Rat IgG2b/IgG1 | FITC PE | flow cytometry | 2 x 1.0 ml; 200 tests |
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M011-0 | GPVI | JAQ1 | Rat IgG2a | purif. | IP, IHC, WB | 0.1 mg |
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M011-1 | GPVI | JAQ1 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M021-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg |
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M021-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M022-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.A1 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M023-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | JON/A | Rat IgG2b | PE | FC (activ. form) | 1.5 ml; 300 tests |
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M024-0 | Integrin alphaIIb (GPIIb, CD41) | MwReg30 | Rat IgG1 | purif. | IP, IHC, generation of Fab fragments | 6 mg |
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M025-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | purif. | IP, IHC | 0.5 mg |
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M025-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M025-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | PE | FC, IHC | 1.5 ml; 300 tests |
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M025-3 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | DyLight 649 | FC, IHC | 1.5 ml; 300 tests |
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M030-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.A5 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
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M031-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | purif. | IP, IHC, WB | 0.5 mg |
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M031-1 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M040-0 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | purif. | IP, IHC | 0.5 mg |
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M040-1 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M040-2 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | PE | FC, IHC | 1.5 ml; 300 tests |
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M040-3 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | DyLight 649 | FC, IHC | 1.5 ml; 300 tests |
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M041-0 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | purif. | FC, IHC | 0.5 mg |
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M041-1 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M042-0 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg |
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M042-1 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M043-0 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | purif. | IP, IHC, block | 0.5 mg |
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M043-1 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M050-0 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
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M050-1 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M051-0 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M051-1 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M060-0 | GPV (CD42d) | Gon.C2 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M060-1 | GPV (CD42d) | Gon.C2 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M061-1 | GPV (CD42d) | Gon.G6 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M070-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg |
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M070-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M071-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | purif. | IP, IHC | 0.5 mg |
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M071-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M075-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1, Sam.G4, Sam.H9 | Rat IgG2b/IgG1 | purif. | IP, IHC | 0.5 mg |
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M080-0 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M080-1 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M110-0 | CD9 | Nyn.H3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
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M110-1 | CD9 | Nyn.H3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M120-0 | CD31 (PECAM-1) | Pec.H3 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg |
| ||||||
M130-0 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M130-1 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M130-2 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | PE | FC, IHC | 1.5 ml; 300 tests |
| ||||||
P140-1 | fibrinogen | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
P150-1 | von Willebrand Factor (vWF) | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
P180-1 | negative control | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.0 ml; 200 tests |
| ||||||
P190-1 | negative control | polyclonal | Rat IgG | FITC | FC, IHC | 1.0 ml; 200 tests |
| ||||||
P190-2 | negative control | polyclonal | Rat IgG | PE | FC, IHC | 1.0 ml; 200 tests |
| ||||||
P190-3 | negative control | polyclonal | Rat IgG | DyLight 649 | FC, IHC | 1.0 ml; 200 tests |
| ||||||
R300 | Antibodies for Mouse Plaet Depletion | polyclonal anti-GPIb alpha | Rat IgG | purif. | plaet depletion | 0.5 mg |
| ||||||
X488 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 488 | in vivo | 100 µg |
| ||||||
X649 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 649 | in vivo | 100 µg |
货号 | 抗原类型 | 克隆方式 | 表型 | 产品形式 | 应用 | 规格 | ||
C301 | 清除小鼠血小板 | polyclonal non-immune rat immunoglobulins | Rat IgG | 纯化 | 清除小鼠血小板 | 0.5 mg | ||
D200 | 双色分析血小板活性 | Wug.E9 JON/A | Rat IgG2b/IgG1 | FITC、PE | 流式细胞术 | 2 x 1.0 ml; 200 tests | ||
M011-0 | GPVI | JAQ1 | Rat IgG2a | 纯化 | IP, IHC, WB | 0.1 mg | ||
M011-1 | GPVI | JAQ1 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M021-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg | ||
M021-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M022-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.A1 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M023-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | JON/A | Rat IgG2b | PE | FC (activ. form) | 1.5 ml; 300 tests | ||
M024-0 | Integrin alphaIIb (GPIIb, CD41) | MwReg30 | Rat IgG1 | purif. | IP, IHC, generation of Fab fragments | 6 mg | ||
M025-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | purif. | IP, IHC | 0.5 mg | ||
M025-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M025-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | PE | FC, IHC | 1.5 ml; 300 tests | ||
M025-3 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | DyLight 649 | FC, IHC | 1.5 ml; 300 tests | ||
M030-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.A5 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M031-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | purif. | IP, IHC, WB | 0.5 mg | ||
M031-1 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M040-0 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | purif. | IP, IHC | 0.5 mg | ||
M040-1 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M040-2 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | PE | FC, IHC | 1.5 ml; 300 tests | ||
M040-3 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | DyLight 649 | FC, IHC | 1.5 ml; 300 tests | ||
M041-0 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | purif. | FC, IHC | 0.5 mg | ||
M041-1 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M042-0 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg | ||
M042-1 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M043-0 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | purif. | IP, IHC, block | 0.5 mg | ||
M043-1 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M050-0 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M050-1 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M051-0 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
| ||||||||
M051-1 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M060-1 | GPV (CD42d) | Gon.C2 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M061-1 | GPV (CD42d) | Gon.G6 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M070-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg | ||
M070-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M071-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | purif. | IP, IHC | 0.5 mg | ||
M071-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M075-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1, Sam.G4, Sam.H9 | Rat IgG2b/IgG1 | purif. | IP, IHC | 0.5 mg | ||
M080-0 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M080-1 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M110-0 | CD9 | Nyn.H3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M110-1 | CD9 | Nyn.H3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M120-0 | CD31 (PECAM-1) | Pec.H3 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg | ||
M130-0 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M130-1 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M130-2 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | PE | FC, IHC | 1.5 ml; 300 tests | ||
P140-1 | fibrinogen | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests | ||
P150-1 | von Willebrand Factor (vWF) | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests | ||
P180-1 | negative control | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.0 ml; 200 tests | ||
P190-1 | negative control | polyclonal | Rat IgG | FITC | FC, IHC | 1.0 ml; 200 tests | ||
P190-2 | negative control | polyclonal | Rat IgG | PE | FC, IHC | 1.0 ml; 200 tests | ||
| ||||||||
P190-3 | negative control | polyclonal | Rat IgG | DyLight 649 | FC, IHC | 1.0 ml; 200 tests | ||
R300 | Antibodies for Mouse Plaet Depletion | polyclonal anti-GPIb alpha | Rat IgG | purif. | plaet depletion | 0.5 mg | ||
X488 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 488 | in vivo | 100 µg | ||
X649 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 649 | in vivo | 100 µg | ||
我们公司优势是强大的采购，
1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，
2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，
3：提供加急服务，一般1-2周到货，超过时限加急费全免
4：价格公道，绝大部分价格有优势，当然不能保证100%产品都是价格低廉,因为价格低廉意味着没有服务.
5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大 交大 复旦，中山等100多所大学，ROCHE，阿斯利康，国药 ，fisher等500多家公司
6：我们*的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，chronolog,Inc，chronolog,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等
7：我们还是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批发，欢迎合作。
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