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美国MEFs Oct4-GFP报告基因小鼠胚胎成纤维细胞
美国PrimCells干细胞：PrimCells提供多种报告基因敲进的小鼠胚胎干细胞，该类细胞皆在原始基因座上敲入荧光报告基因，从而使荧光蛋白的表达能真实反映原基因座附近的表达调控状况。该类细胞作为研究胚胎干细胞自更新和定向分化的有力工具，受到多家有名研究所研究人员的青睐。
| Species: | Mus Musculus (129/B6 Mix) | Catalog Number: | PCEMM03 |
| Cell Number: | ~1x106 | Storage/Shipping: | Dry Ice |
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美国MEFs Oct4-GFP报告基因小鼠胚胎成纤维细胞
Introduction:
Primary mouse embryonic fibroblasts (MEFs) are typically derived from E13.5 mouse embryos. They are relatively easy to culture and maintain and have robust proliferation rate. MEFs can be powerful tools to study gene functions especially when comparison experiments between wild-type and knockout cells from the same liter are required. Meanwhile, MEFs have also been used as feeder cells to support growth of embryonic stem cells from a variety of species1. More recently, MEFs from reporter transgenic mice were used for assessing gene function during reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) 2.
PrimCells is proud to isolate and provide the primary cultures of embryonic fibroblasts from transgenic mice carrying IRES-GFP gene in their endogenous Pou5f1 (Oct4) locus. When these cells are fully reprogrammed, iPSCs will become GFP+, which could be a powerful tool for chemical screening or gene functional studies in order to develop more efficient reprogramming technology.
Figure 1 | Successfully reprogrammed iPSCs become GFP positive
美国PrimCells品牌介绍
PrimCells是位于美国加州圣迭戈的一家专业致力于细胞产品研发和解决方案提供的业内技术。
公司汇集了*的生物医药技术专家，独立开发了多项原代细胞和干细胞相关的专有技术。 公司以“细胞研究的优选择"为宗旨，自主研发超过70种创新产品，多个特种细胞市场的空白。例如其诱导性表达细胞库和高效3D皮肤培养系统，皆为该类产品目前市场上少数的规模化供应商。此外，公司以高质量的数据与快速稳定的项目完成时间，长期为多家制药公司提供解决方案，获得用户广泛赞誉。
PrimCells产品线：
1. 报告基因干细胞 2. 原代细胞（含报告基因原代细胞） 3. 诱导性稳定细胞株
4. 人源重组蛋白 5. 创新型培养试剂
PrimCells 解决方案
1.诱导性稳定细胞株开发 2. 原代细胞开发 3. 药物体外毒理和效能测试 4. 3D-皮肤培养
PrimCells合作伙伴及客户:
A．生物制药公司：辉瑞(Pfizer), 诺华(Novartis), 礼来(Eli2 Lilly),. Amgen, EMD Millipore, Regeneron等公司的毒理和医药研发部门
B．科研机构：冷泉港实验室，哈佛医学院, 麻省理工（MIT）,， UCSF, NYU, Salk, UIUC, USC等
