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蛋白凝胶电泳及相关的Western-Blot是蛋白分析的主流技术 (Figure 1)。Applied BioProbes 公司提供两种蛋白染料： 1)Coomassie® Blue Fast Stain, 一种即用型的蛋白染料，无需甲醇或乙酸固定、或脱色； 2) eLuminol™ Protein Gel Stain，一种具有*灵敏度的蛋白荧光染料，用于检测SDS-PAGE凝胶电泳中的总蛋白。Applied BioProbes 公司也提供一种专门检测磷酸化蛋白的荧光染料： Phos-Tag™ Phosphoprotein Gel Stain。Applied BioProbes提供的 ECL Western Blotting Detection Kit用于WB检测HRP标记的抗体及其关联的抗原，具有快速检测、灵敏度*等特点。另外，Applied BioProbes也提供了两种蛋白定量分析试剂盒： 1) Bradford Protein Assay Kit；和 2) BCA Protein Assay Kit。

Figure 1. Protein gel analysis workflow

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Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), and Phos-Tag™ Phosphoprotein Gel Stain, followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.

Figure 3. Detection of protein in Western Blots with ABP kit (left) and Millipore Kit (right).

Figure 4. BSA standard curve with Bradford Protein Assay Kit (left) and BCA Protein Assay Kit (right).