亚洲精品综合日韩中文字幕网站_精品综合久久久久97_中文在线天堂网www_久久精品免费一区二区三区_91久久国产综合精品女同国语_久久资源总站在线国产成人

上海起發(fā)實驗試劑有限公司

中級會員·16年

聯(lián)系電話

15921799099

您現(xiàn)在的位置: 首頁> 資料下載> pCMV-Tag 2B載體說明書

pCMV-Tag 2B載體說明書

2013-9-6  閱讀(3427)

分享:
提  供  商 上海起發(fā)實驗試劑有限公司 資料大小 319.5KB
資料類型 PDF 文件 資料圖片
下載次數(shù) 220次 瀏覽次數(shù) 3427次
型號載體名稱出品公司載體用途價格
VPS0040pCMV-Tag 2BStratagene哺乳動物表達(dá)載體800

 

產(chǎn)品參數(shù): 

Key Function:Constitutive Expression
Mammalian Selection:Neo,G418
Promoter:CMV
Cloning Method:Restriction Enzyme/MCS
Constitutive or Inducible System:Constitutive

載體抗性: 

卡那霉素(Kanamycin)

載體描述: 

Epitope tags provide a method to localize gene products in living cells, identify associated proteins, track the movement of tagged proteins within a cell, and characterize new genes without creating protein-specific antibodies. The pCMV-Tag1 vector contains both the synthetic FLAG epitopell ll and the c-myc epitope from the human c-myc gene. The pCMV-Tag1 vector allows production of fusion proteins in a variety of conformations. The pCMV-Tag2-5 vectors allow either c-myc or FLAG epitope tagging at either the C- or N-terminus. Each pCMV-Tag2-5 vector is supplied with all three reading frames for easy cloning and expression. The small size of the FLAG (eight amino acids long) and c-myc epitope tags (ten amino acids long) decreases the possibility of interference with the tagged protein. The FLAG and c-myc epitopes are recognized by the anti-c-myc and anti-FLAG antibodies, which can be used to characterize the target protein. The pCMV-Tag vectors allow high-level expression of tagged proteins in mammalian cells. Expression is driven by the human cytomegalovirus (CMV) immediate early promoter. The CMV promoter provides constitutive expression of cloned genes in a wide variety of cell lines. The translational start sequence used in the pCMV-Tag1, pCMV-Tag2 and pCMV-Tag3 vectors is a 10-base Kozak consensus sequence of CGG(A/G)CCATGG. pCMV-Tag4 and pCMV-Tag5 vectors do not contain a translation start sequence. Stable clone selection is made possible with G418 by the presence of the neomycin-resistance gene. The pCMV-Tag vectors are only 4.3 kb, allowing easy cloning and vector manipulations. This small size is due to the single antibiotic selection marker used by both prokaryotic and mammalian cells. The neomycin-resistance gene provides stable selection in mammalian cells with G418 driven by the SV40 promoter and kanamycin selection in E. coli cells driven by the bla (ß-lactamase) promoter.

 

質(zhì)粒圖譜: 

pCMV-Tag2 質(zhì)粒圖譜

上海起福生物科技有限公司
:楊建輝

辦公:
*8006

:
:http://www.qfbio.com/
地址:上海市浦東新區(qū)繡川路561號1102室

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~
產(chǎn)品對比 二維碼 在線交流

掃一掃訪問手機(jī)商鋪

對比框

在線留言
宕昌县| 桐梓县| 碌曲县| 荆门市| 银川市| 石景山区| 龙门县| 大厂| 开鲁县| 三原县| 烟台市| 抚顺市| 余干县| 镇巴县| 怀安县| 定西市| 克拉玛依市| 五河县| 泽州县| 蒙山县| 蒙城县| 白银市| 颍上县| 白玉县| 新田县| 宽城| 滨州市| 桃园县| 宽城| 驻马店市| 罗城| 措美县| 四子王旗| 奇台县| 阿拉善右旗| 黄大仙区| 荃湾区| 乌拉特中旗| 定边县| 晋州市| 昔阳县|