亚洲精品综合日韩中文字幕网站_精品综合久久久久97_中文在线天堂网www_久久精品免费一区二区三区_91久久国产综合精品女同国语_久久资源总站在线国产成人

上海起發(fā)實(shí)驗(yàn)試劑有限公司

中級(jí)會(huì)員·16年

聯(lián)系電話

15921799099

您現(xiàn)在的位置: 首頁(yè)> 資料下載> pCMV-Tag 5B載體說(shuō)明書

pCMV-Tag 5B載體說(shuō)明書

2013-9-6  閱讀(2200)

分享:
提  供  商 上海起發(fā)實(shí)驗(yàn)試劑有限公司 資料大小 319.5KB
資料類型 PDF 文件 資料圖片
下載次數(shù) 197次 瀏覽次數(shù) 2200次
型號(hào)載體名稱出品公司載體用途價(jià)格
VPS0545pCMV-Tag 5BStratagene哺乳動(dòng)物表達(dá)載體800

 

產(chǎn)品參數(shù): 

Key Function:Constitutive Expression
Mammalian Selection:Neo,G418
Promoter:CMV
Cloning Method:Restriction Enzyme/MCS
Constitutive or Inducible System:Constitutive

載體抗性: 

卡那霉素(Kanamycin)

載體描述: 

Epitope tags provide a method to localize gene products in living cells, identify associated proteins, track the movement of tagged proteins within a cell, and characterize new genes without creating protein-specific antibodies. The pCMV-Tag1 vector contains both the synthetic FLAG epitopell ll and the c-myc epitope from the human c-myc gene. The pCMV-Tag1 vector allows production of fusion proteins in a variety of conformations. The pCMV-Tag2-5 vectors allow either c-myc or FLAG epitope tagging at either the C- or N-terminus. Each pCMV-Tag2-5 vector is supplied with all three reading frames for easy cloning and expression. The small size of the FLAG (eight amino acids long) and c-myc epitope tags (ten amino acids long) decreases the possibility of interference with the tagged protein. The FLAG and c-myc epitopes are recognized by the anti-c-myc and anti-FLAG antibodies, which can be used to characterize the target protein. The pCMV-Tag vectors allow high-level expression of tagged proteins in mammalian cells. Expression is driven by the human cytomegalovirus (CMV) immediate early promoter. The CMV promoter provides constitutive expression of cloned genes in a wide variety of cell lines. The translational start sequence used in the pCMV-Tag1, pCMV-Tag2 and pCMV-Tag3 vectors is a 10-base Kozak consensus sequence of CGG(A/G)CCATGG. pCMV-Tag4 and pCMV-Tag5 vectors do not contain a translation start sequence. Stable clone selection is made possible with G418 by the presence of the neomycin-resistance gene. The pCMV-Tag vectors are only 4.3 kb, allowing easy cloning and vector manipulations. This small size is due to the single antibiotic selection marker used by both prokaryotic and mammalian cells. The neomycin-resistance gene provides stable selection in mammalian cells with G418 driven by the SV40 promoter and kanamycin selection in E. coli cells driven by the bla (ß-lactamase) promoter.

 

質(zhì)粒圖譜: 

pCMV-Tag5 質(zhì)粒圖譜

上海起福生物科技有限公司
:楊建輝

辦公:
*8006

:
:http://www.qfbio.com/
地址:上海市浦東新區(qū)繡川路561號(hào)1102室

會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
產(chǎn)品對(duì)比 二維碼 在線交流

掃一掃訪問(wèn)手機(jī)商鋪

對(duì)比框

在線留言
焉耆| 化德县| 开阳县| 海伦市| 轮台县| 桃园县| 兴城市| 屏边| 海城市| 安义县| 南昌县| 榆中县| 阳江市| 雅安市| 莎车县| 犍为县| 长岭县| 通城县| 宝山区| 古田县| 饶平县| 溧水县| 轮台县| 双城市| 米林县| 隆林| 普陀区| 永宁县| 宁海县| 株洲市| 故城县| 都昌县| 宜兴市| 东安县| 清河县| 四川省| 衡阳县| 辛集市| 大冶市| 海门市| 任丘市|