聯(lián)系電話
- 聯(lián)系人:
- 楊經(jīng)理
- 電話:
- 021-50724187 021-50724961
- 手機(jī):
- 15921799099
- 傳真:
- 021-50724961
- 地址:
- 上海浦東川沙鎮(zhèn)川沙路6619號(hào)上海起發(fā)實(shí)驗(yàn)試劑有限公司
- 個(gè)性化:
- www.qfbio.com
- 網(wǎng)址:
- www.qfbio.com
掃一掃訪問手機(jī)商鋪
RNAi載體,RNAi-Ready pSIREN-Retro Q-ZsGreen載體
RNAi-Ready pSIREN-Retro Q-ZsGreen
型號(hào) | 載體名稱 | 出品公司 | 載體用途 |
VRC0347 | RNAi-Ready pSIREN-Retro Q-ZsGreen | Clontech | RNAi載體 |
Promoter: Human U6
Reporter: ZsGreen
Selection: prokaryotic—ampicillin
Replication: prokaryotic—Col E1 ori (low copy), viral—SV40 ori
RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.
