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RNAi載體,RNAi-Ready pSIREN-Retro Q-DsRed-Express載體
RNAi-Ready pSIREN-Retro Q-DsRed-Express
型號 | 載體名稱 | 出品公司 | 載體用途 |
VRC0346 | RNAi-Ready pSIREN-Retro Q-DsRed-Express | Clontech | RNAi載體 |
RNAi-Ready pSIREN-RetroQ-DsRed-Express is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter (PU6; RNA Pol III-dependent). RNAi-Ready pSIREN-RetroQ-DsRed-Express is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQDsRed-Express construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA.
