聯(lián)系電話
上海起發(fā)實(shí)驗(yàn)試劑有限公司
中級(jí)會(huì)員·16年
- 聯(lián)系人：
- 楊經(jīng)理
- 電話：
- 021-50724187 021-50724961
- 手機(jī)：
- 15921799099
- 傳真：
- 021-50724961
- 地址：
- 上海浦東川沙鎮(zhèn)川沙路6619號(hào)上海起發(fā)實(shí)驗(yàn)試劑有限公司
- 個(gè)性化：
- www.qfbio.com
- 網(wǎng)址：
- www.qfbio.com
掃一掃訪問(wèn)手機(jī)商鋪
 提 供 商 |
上海起發(fā)實(shí)驗(yàn)試劑有限公司 |  資料大小 |
273.4KB |
|---|---|---|---|
 資料類型 |
PDF 文件 |  資料圖片 |
|
 下載次數(shù) |
388次 |  瀏覽次數(shù) |
3225次 |
Clontech AH109說(shuō)明書(shū)，AH109說(shuō)明書(shū)，Clontech AH109
AH109
| 型號(hào) | 載體名稱 | 出品公司 | 載體用途 |
| VNC0491 | AH109 | Clontech | 配套釀酒酵母 |
his section provides detailed phenotypes of the yeast strains included with MATCHMAKER System 3
and MATCHMAKER GAL4 cDNA and Genomic Libraries. For additional information on the growth and
maintenance of yeast, see the YPH, Chapter III. We also recommend Guthrie & Fink’s Guide to Yeast
Genetics and Molecular Biology （1991） and Heslot & Gailardin’s Molecular Biology and Genetic
Engineering of Yeasts （1992）.
A. Yeast Host Strains
The complete genotypes of AH109, Y187, and CG-1945 are provided in Table II. All strains are
gal4
–
and gal80
–
; this prevents interference of native regulatory proteins with the regulatory
elements in the two-hybrid system.
1. Use AH109 as the host strain if you plan to screen an AD/library using HIS3, ADE2, and MEL1.
2. System 3 Users Only: Use Y187 as the host strain if you plan to test for an interaction between
two known proteins using the lacZ reporter only. In addition, use Y187 as a mating partner to
verify protein interactions.
3. Library Users Only: Use CG-1945 as the host strain if you plan to separate DNA/bait and
AD/library plasmids by cycloheximide counterselection. Alternatively, use pGBKT7 to construct your bait. This DNA-BD vector contains a kanamycin resistance marker; therefore,
vectors can be separated in E. coli without cycloheximide counterselection.
