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Matchmaker™ Library Construction & Screening Kits (pGBKT7,pGADT7,pGBKT7-53,pGADT7-T,pGBKT7-Lam)

2013-7-23  閱讀(6611)

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pGBKT7-53

 
產(chǎn)品參數(shù): 

Bacteria Resistance:KanamycinMammalian Selection:-

載體抗性: 
卡那霉素(Kanamycin)
載體描述: 

載體特點(diǎn):與pGADT7-T構(gòu)成Matchmaker Two-Hybrid System 3的雙雜交正對(duì)照。

pGBKT7-53 encodes a fusion of the murine p53 protein (a.a. 72–390) and the GAL4 DNA-BD (a.a. 1–147). The murine p53 cDNA (GenBank Accession #K01700) was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. (1993); plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.

質(zhì)粒圖譜: 
pGBKT7-53 質(zhì)粒圖譜

 

pGBKT7-Lam

產(chǎn)品參數(shù): 

Bacteria Resistance:KanamycinMammalian Selection:-

載體抗性: 
卡那霉素(Kanamycin)
載體描述: 

載體特點(diǎn):Matchmaker Two-Hybrid System 3的負(fù)對(duì)照。

pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bar et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.

質(zhì)粒圖譜: 
pGBKT7-Lam 質(zhì)粒圖譜

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