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IL 17 Mouse/重組小鼠白介素17

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IL 17 Mouse/重組小鼠白介素17
產(chǎn)品編號(hào)：CYT-378
產(chǎn)品規(guī)格：5μg/25μg/1mg

Synonyms	CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.
Introduction	IL17 is a proinflammatory cytokine produced by activated T cells. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. Interleukin-17 can stimulate the expression of IL6 and cyclooxygenase-2 （PTGS2/COX-2）, as well as enhance the production of nitric oxide （NO）. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Description	Interleukin-17 Murine Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 266 amino acids and having a molecular mass of 29,956 Dalton. The IL-17 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	Lyophilized from a concentrated （1mg/ml） solution containing no additives.
Solubility	It is recommended to reconstitute the lyophilized Interleukin 17 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Interleukin-17 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL17 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Amino acid sequence	The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ala-Ile-Ile-Pro.
Biological Activity	The ED50 as determined by the dose-dependant secretion of IL-6 by NIH 3T3 cells was found to be 1.0-10.0 ng/mL.
Protein content	Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.942 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.