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GRO b Mouse/ 重組小鼠生長(zhǎng)相關(guān)基因β（CXCL2）

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ELISA試劑盒: 人的ELISA試劑盒小鼠的ELISA試劑盒大鼠的ELISA試劑盒雞的ELISA試劑盒牛的ELISA試劑盒其它的ELISA試劑盒植物ELISA試劑盒豬的ELISA試劑盒兔的ELISA試劑盒

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GRO b Mouse/ 重組小鼠生長(zhǎng)相關(guān)基因β（CXCL2）
產(chǎn)品編號(hào)：CHM-338
產(chǎn)品規(guī)格：5μg/20μg/1mg

Synonyms	Macrophage inflammatory protein 2-alpha, MIP2-alpha, CXCL2, Growth- regulated protein beta, Gro-beta, chemokine （C-X-C motif） ligand 2, GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, MGSA beta.
Introduction	Chemokine （C-X-C motif） ligand 2 （CXCL2） is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha （MIP2-alpha）, Growth-regulated protein beta （Gro-beta） and Gro oncogene-2 （Gro-2）. CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.
Description	GRO-Beta Mouse Recombinant also caled mouse MIP-2 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7849 Dalton. The CXCL2 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	The protein was lyophilized with no additives.
Solubility	It is recommended to reconstitute the lyophilized GRO-beta Mouse in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized CXCL2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Amino acid sequence	The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Val-Ala-Ser.
Biological Activity	The Biological activity was determined by measuring the dose dependent mobilization of intracellular calcium （calcium flux） with human neutrophils. Significant calcium mobilization is observed with >5 ng/ml of recombinant mouse MIP-2.
Protein content	Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.015 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of GRO-b as a Reference Standard.
Usage	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.