pierce SuperSignal West Pico Chemiluminescent Substrate pierce SuperSignal West Pico Chemiluminescent Substrate trate

产品品牌:pierce 英文名称:SuperSignal West Pico Chemiluminescent Substrate 产品货号:34079 产品规格:50ml 

描述

Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for low-picogram-level detection by Western blot analysis.
SuperSignal West Pico Substrate provides intense signal and picogram sensitivity for Western blotting with horseradish peroxidase (HRP) enzyme conjugates. The ECL substrate is compatible with different membranes, blocking reagents and a wide range of antibody dilutions. SuperSignal West Pico Substrate provides excellent performance, versatility and economy for routine Western blotting needs.
Features of SuperSignal West Pico Chemiluminescent Substrate:
• ECL—enhanced chemiluminescent substrate for horseradish peroxidase (HRP)
• Picogram sensitivity—detect low-picogram amounts of protein in bands on nitrocellulose or PVDF membrane
• Good signal duration—incubated blots provide 6 to 8 hours of usable light output when conditions are optimized
• Stable reagent—24-hour working solution stability; 1-year kit stability at room temperature
• Economical—optimized for dilute antibody concentrations:
• 0.2 to 1.0 µg/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock)
• 10 to 50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock)
For Research Use Only. Not for use in diagnostic procedures.

规格

内容及储存

Sufficient For: 6 mini-gel blots; 500 cm2 of membrane
• Luminol/Enhancer, 25 mL
• Stable Peroxide Buffer, 25 mL

Store at room temperature.