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北京西美杰科技有限公司
主营产品： 生物学检测试剂盒,化学试剂,蛋白质氧化损伤,抗原抗体 |
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您现在的位置： 北京西美杰科技有限公司>>分子生物学试剂>> A5253,0025DNA Swab Isolation Spin-Kit（咽试子DNA提取试剂盒）
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- 西美杰
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A5253,0025DNA Swab Isolation Spin-Kit（咽试子DNA提取试剂盒）
| 参考价 | 面议 |
具体成交价以合同协议为准
- 型号 A5253,0025
- 品牌
- 厂商性质 代理商
- 所在地 上海市
更新时间：2017-07-19 20:19:26浏览次数：819
联系我们时请说明是化工仪器网上看到的信息，谢谢!
| HS-No. | 38220000 | |||
|---|---|---|---|---|
| Storage | RT | |||
| except for Proteinase K - Solution: +4°C | ||||
| • Kit for the DNA isolation from swabs with spin minicolumns | ||||
| • Advantage: approx. preparation time 30 minutes | ||||
| • Binding capacity of the column 10 μg DNA | ||||
| • sufficient for 25 and 100 isolations, respectively | ||||
This kit is based on the property of DNA to adsorb to silica surfaces in the presence of high concentrations of chaotropic salts. The lysis of cells from swabs is performed in the lysis solution containing chaotropic salts and nonionic detergents. The lysis process is supported by auxiliary, high affinity protease （Proteinase K）. Such reagents assure lysis and complete protein degradation. Subsequently the Iysate is loaded onto the spin minicolumn with dedicated silica membrane. The DNA binds to the silica membrane, while contaminants （fragmented proteins, salts. etc.） pass through the column freely. Residual contaminants are moved away by a wash step. Pure DNA is eluted with a low salt buffer or water and can be used in following experiments without further purification procedures （e.g. precipitation）.
