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SpeciesReactivity
Mouse

Form
Stds=10-200 ng/ml; Sample=100 ul (diluted); Sensitivity= 5 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Anti-Mouse IgG1 coated strip plate (8 wells x 12 strips) 6331 2. Mouse IgG1 Std. A (0 ng/ml), 0.22 ml 6332 3. IgG1 Std B (50 ng/ml), 0.22 ml 6333 4. IgG1 Std C (100 ng/ml), 0. 22 ml 6334 5. IgG1 Std D (300 ng/ml), 0. 22 ml 6335 6. IgG1 Std E (600 ng/ml), 0. 22 ml 6336 7. IgG1 Std F (900 ng/ml), 0. 22 ml 6337 8. Wash Buffer (100X), 10 ml WB-100 9. Sample Diluent (20x), 10 ml SD-20T 10. Anti-mouse IgG1-HRP Conjugate (100x), 0.12 ml 6338 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Instruction Manual M-6330

SpCrossreactivity
The antibodies used in this kit are specific for IgG1 with no significant reactivity to IgG2-3 or IgA, IgM, or IgE. Mouse IgG1 ELISA kit species crossreactivity was tested with the following animal serum (IgG1) at dilutions of 1:500: No significant crossreactivity was observed with Rat, Hamster, Guinea Pig, Rabbit, Human, Monkey, Goat, Sheep, FBS, Bovine, Pig, and Chicken serum IgG1.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.