M00091 Streptavidin-HRP
| 参考价 | ￥ 890 |
| 订货量 | ≥1件 |
- 公司名称 漠宝（厦门）生物科技有限公司
- 品牌 GenScript/金斯瑞
- 型号 M00091
- 产地
- 厂商性质 代理商
- 更新时间 2025/7/22 10:32:15
- 访问次数 41
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| 供货周期 | 一周 | 规格 | 1 mg |
|---|---|---|---|
| 货号 | M00091 | 应用领域 | 医疗卫生,环保,食品/农产品,生物产业,制药/生物制药 |
| 主要用途 | 是将链霉亲和素与辣根过氧化物酶（HRP）偶联而成的 |
Streptavidin-HRP (M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.
产品概述
Product Description
Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces Avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for biotin.
Streptavidin-HRP (M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.
产品属性
Formulation ：Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal
Reconstitution： Reconstitute the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final concentration of 1 mg/ml.
Storage： The product remains stable in lyophilized form for at least 1 year if stored at 2-8°C, -20°C, or lower from the date of receipt. For longer storage, -20°C or below is recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for up to 1 year at -20°C or below. Avoid repeated freezing and thawing cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.
应用指导
Working concentrations for specific applications should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time, etc. The following dilution ranges are just a guideline:
Application： Recommended Usage
Dot blot ：1:2,000~1:10,000
Western blot： 1:2,000~1:20,000
ELISA ：1:5,000~1:20,000
Others： User optimized
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