UM-UC-3人膀胱移行细胞癌
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人膀胱移行细胞癌UM-UC-3
| 种属 | 人 |
| 别称 | UMUC-3; UM-UC3; UMUC3; UC-3; University of Michigan-Urothelial Carcinoma-3 |
| 组织来源 | 膀胱 |
| 疾病 | 移行细胞癌 |
| 传代比例/细胞消化 | 1:2传代 ，消化2-3分钟 |
| 培养基配置 | MEM培养基；10%胎牛血清；1%双抗 |
| 简介 | Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) ａnd others. The X and N3 had single copy per cell, and others were generally two or three copies per cell. |
| 形态 | 上皮细胞样 |
| 生长特征 | 贴壁生长 |
| 倍增时间 | 每周 2 至 3 次 |
| 致瘤性 | Yes, Tumors developed within 21 days at 99% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| STR | Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 8,9 D5S818: 12 D7S820: 8,9 THO1: 6,9 TPOX: 10 vWA: 17 |
| 培养条件 | 气相 ：空气 ，95% ；二氧化碳 ，5%。 温度 ：37摄氏度 ，培养箱湿度为70%-80%。 |
| 冻存条件 | 冻存液 ：90%FBS ，DMSO 10%, 或使用非程序冻存液 ：货号JY-H040 |
| 保藏机构 | ATCC; CRL-1749 |
| 产品使用 | 于科学研究 ，不可作为动物或人类疾病的治疗产品使用。 |
Compared with the control group，the expression levels of Afamin and DBP in serum of the POI group were significantly increased (P＜0．05) ; the expression levels of serum Afamin and DBP were positively correlated with FSH and E2 (P＜ 0．05) ，but negatively correlated with AMH (P＜0. 05) ;
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