3D Cell Culture Chip 三维细胞培养芯片

材料

芯片采用生物相容性聚二甲基硅氧烷（PDMS）制成，具有透气性，保证了CO2和O2的交换薄玻璃载体是一种具有高透明、低自荧光等优良光学性能的显微镜载玻片。这允许通过光学显微镜、荧光显微镜和共焦显微镜以时间和空间分辨率直接观察生物样品。

技术特点

边沟中间沟

高度150微米150微米

宽度

3250微米

1125微米（狭窄部分）

3375微米（宽部分）

体积（建议注射）

20微升20微升

入口直径1000微米1000微米

芯片表面没有涂层。根据您的应用，并终改善细胞粘附性，您可以使用聚合物和/或蛋白质溶液来覆盖通道表面。

这种微流控装置还可以与细胞嵌入水凝胶（如I型胶原、IV型胶原、纤维蛋白、基质凝胶等）中，以重建可靠的生物微环境。

入口直径为1毫米，可通过普通吸管轻松注入细胞悬液20微升是每个通道/腔室的推荐体积；这将允许在实际注射和*填充时有少量额外的微升。

此外，还可以使用直接安装在入口的1/16“管子在动态流动条件下进行研究。

好处

使用方便

生理相关微环境

单细胞分辨率

直接可视化

时空分辨率

定性和定量评估

3D Cell Culture Chip 三维细胞培养芯片

Materials

The IC-Chip is made of biocompatible polydimethylsiloxane (PDMS) and is gas-permeable to ensure the CO2 and O2 exchange. The support in thin glass is a microscope slide with excellent optical properties such as high transparency and low autofluorescence. This allows a direct observation of your biological sample with temporal and spatial resolution by using optical, fluorescent and confocal microscopes.

Technical features

The IC-Chip has not coated surfaces. According to your application and to eventually improve cell adhesion, you can coat the channel surface by using your solutions of polymers and/or proteins. 

This microfluidic device can be used also with cells embedded in hydrogels such as Collagen I, Collagen IV, Fibrin, Matrigel, etc. to recreate a reliable biological microenvironment. 

The inlet diameter of 1 mm allows an easy injection of the cell suspension through common pipettes. 20 µL is the recommended volume for each channel/chamber; this will allow to have few extra µL for a practical injection and complete filling.

Additionally, it would be possible to perform studies in dynamic flow conditions by using 1/16” tubing directly fitted in the inlets. 

Benefits

• Easy-to-use
• Physiologically relevant microenvironment 
• Single cell resolution 
• Direct visualization
• Temporal and spatial resolution 
• Qualitative and quantitative assessments